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1000
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Abstract/Summary
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The working group „Analyses in Biological Materials“ of the Permanent Senate Commission for the Investigation of Health Hazards of Chemical Compounds in the Work Area developed and validated the presented biomonitoring method. The analytical method described hereinafter permits the selective detection of 4‐tert‐octylphenol as well as the sum of various branched p‐nonylphenol isomers in urine. After adding the labelled internal standards (13C6‐4‐tert‐octylphenol and 13C6‐p‐nonylphenol), the samples are enzymatically hydrolysed to release the analytes from the conjugated alkylphenols. After online SPE, the analytes are separated by liquid chromatography and analysed using tandem mass spectrometry. A quantitation limit of 2 µg/L each is obtained for the analytes. Calibration standards are prepared in pooled urine and processed in the same way as the samples to be analysed.
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