Human neuronal signaling and communication assays to assess functional neurotoxicity

  1. Loser, Dominik ORCID logo
  2. Schaefer, Jasmin
  3. Danker, Timm
  4. Möller, Clemens
  5. Brüll, Markus
  6. Suciu, Ilinca
  7. Ückert, Anna-Katharina
  8. Klima, Stefanie
  9. Leist, Marcel
  10. Kraushaar, Udo

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WeightNameValue
1000 Titel
  • Human neuronal signaling and communication assays to assess functional neurotoxicity
1000 Autor/in
  1. Loser, Dominik |
  2. Schaefer, Jasmin |
  3. Danker, Timm |
  4. Möller, Clemens |
  5. Brüll, Markus |
  6. Suciu, Ilinca |
  7. Ückert, Anna-Katharina |
  8. Klima, Stefanie |
  9. Leist, Marcel |
  10. Kraushaar, Udo |
1000 Erscheinungsjahr 2020
1000 Publikationstyp
  1. Artikel |
1000 Online veröffentlicht
  • 2020-12-02
1000 Erschienen in
1000 Quellenangabe
  • 95(1):229-252
1000 Copyrightjahr
  • 2020
1000 Lizenz
1000 Verlagsversion
  • https://doi.org/10.1007/s00204-020-02956-3 |
  • https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7811517/ |
1000 Publikationsstatus
1000 Sprache der Publikation
1000 Abstract/Summary
  • Prediction of drug toxicity on the human nervous system still relies mainly on animal experiments. Here, we developed an alternative system allowing assessment of complex signaling in both individual human neurons and on the network level. The LUHMES cultures used for our approach can be cultured in 384-well plates with high reproducibility. We established here high-throughput quantification of free intracellular Ca
1000 Sacherschließung
lokal High-Throughput Screening Assays [MeSH]
lokal Neurotoxicity Syndromes/etiology [MeSH]
lokal Purinoceptor
lokal Action Potentials/drug effects [MeSH]
lokal Receptors, Purinergic/metabolism [MeSH]
lokal Neurons/drug effects [MeSH]
lokal Toxicity Tests [MeSH]
lokal Neuronal network
lokal Voltage-Gated Sodium Channels/metabolism [MeSH]
lokal Calcium Signaling/drug effects [MeSH]
lokal Calcium/metabolism [MeSH]
lokal Dopamine Plasma Membrane Transport Proteins/drug effects [MeSH]
lokal Dopamine transporter
lokal Humans [MeSH]
lokal Neurons/metabolism [MeSH]
lokal Receptors, Purinergic/drug effects [MeSH]
lokal Organ Toxicity and Mechanisms
lokal Nerve Net/drug effects [MeSH]
lokal Time Factors [MeSH]
lokal Neurotoxicity Syndromes/pathology [MeSH]
lokal Nerve Net/metabolism [MeSH]
lokal Receptors, Purinergic/genetics [MeSH]
lokal Nerve Net/pathology [MeSH]
lokal Network oscillations
lokal Voltage-Gated Sodium Channels/drug effects [MeSH]
lokal Neurons/pathology [MeSH]
lokal Neurotoxicity Syndromes/metabolism [MeSH]
lokal Cells, Cultured [MeSH]
lokal Dopamine Plasma Membrane Transport Proteins/metabolism [MeSH]
lokal Channel toxins
lokal Patch-Clamp Techniques [MeSH]
1000 Liste der Beteiligten
  1. https://orcid.org/0000-0002-2513-238X|https://frl.publisso.de/adhoc/uri/U2NoYWVmZXIsIEphc21pbg==|https://frl.publisso.de/adhoc/uri/RGFua2VyLCBUaW1t|https://frl.publisso.de/adhoc/uri/TcO2bGxlciwgQ2xlbWVucw==|https://frl.publisso.de/adhoc/uri/QnLDvGxsLCBNYXJrdXM=|https://frl.publisso.de/adhoc/uri/U3VjaXUsIElsaW5jYQ==|https://frl.publisso.de/adhoc/uri/w5xja2VydCwgQW5uYS1LYXRoYXJpbmE=|https://frl.publisso.de/adhoc/uri/S2xpbWEsIFN0ZWZhbmll|https://frl.publisso.de/adhoc/uri/TGVpc3QsIE1hcmNlbA==|https://frl.publisso.de/adhoc/uri/S3JhdXNoYWFyLCBVZG8=
1000 Hinweis
  • DeepGreen-ID: 28aa7d918c1342d290186faad7e72e0f ; metadata provieded by: DeepGreen (https://www.oa-deepgreen.de/api/v1/), LIVIVO search scope life sciences (http://z3950.zbmed.de:6210/livivo), Crossref Unified Resource API (https://api.crossref.org/swagger-ui/index.html), to.science.api (https://frl.publisso.de/), ZDB JSON-API (beta) (https://zeitschriftendatenbank.de/api/), lobid - Dateninfrastruktur für Bibliotheken (https://lobid.org/resources/search)
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1000 Dateien
1000 Objektart article
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1000 @id frl:6466372.rdf
1000 Erstellt am 2023-11-16T22:35:42.682+0100
1000 Erstellt von 322
1000 beschreibt frl:6466372
1000 Zuletzt bearbeitet 2023-12-01T04:08:05.800+0100
1000 Objekt bearb. Fri Dec 01 04:08:05 CET 2023
1000 Vgl. frl:6466372
1000 Oai Id
  1. oai:frl.publisso.de:frl:6466372 |
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