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1000 Titel
  • Besnoitia besnoiti–driven endothelial host cell cycle alteration
1000 Autor/in
  1. Velasquez, Zahady |
  2. Lopez-Osorio, Sara |
  3. Pervizaj-Oruqaj, Learta |
  4. Herold, Susanne |
  5. Hermosilla, Carlos |
  6. Taubert, Anja |
1000 Erscheinungsjahr 2020
1000 Publikationstyp
  1. Artikel |
1000 Online veröffentlicht
  • 2020-06-17
1000 Erschienen in
1000 Quellenangabe
  • 119(8):2563-2577
1000 Copyrightjahr
  • 2020
1000 Lizenz
1000 Verlagsversion
  • https://doi.org/10.1007/s00436-020-06744-x |
  • https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7366594/ |
1000 Publikationsstatus
1000 Sprache der Publikation
1000 Abstract/Summary
  • Besnoitia besnoiti is an important obligate intracellular parasite of cattle which primarily infects host endothelial cells of blood vessels during the acute phase of infection. Similar to the closely related parasite Toxoplasma gondii, B. besnoiti has fast proliferating properties leading to rapid host cell lysis within 24–30 h p.i. in vitro. Some apicomplexan parasites were demonstrated to modulate the host cellular cell cycle to successfully perform their intracellular development. As such, we recently demonstrated that T. gondii tachyzoites induce G2/M arrest accompanied by chromosome missegregation, cell spindle alteration, formation of supernumerary centrosomes, and cytokinesis impairment when infecting primary bovine umbilical vein endothelial cells (BUVEC). Here, we follow a comparative approach by using the same host endothelial cell system for B. besnoiti infections. The current data showed that—in terms of host cell cycle modulation—infections of BUVEC by B. besnoiti tachyzoites indeed differ significantly from those by T. gondii. As such, cyclin expression patterns demonstrated a significant upregulation of cyclin E1 in B. besnoiti–infected BUVEC, thereby indicating parasite-driven host cell stasis at G1-to-S phase transition. In line, the mitotic phase of host cell cycle was not influenced since alterations of chromosome segregation, mitotic spindle formation, and cytokinesis were not observed. In contrast to respective T. gondii–related data, we furthermore found a significant upregulation of histone H3 (S10) phosphorylation in B. besnoiti–infected BUVEC, thereby indicating enhanced chromosome condensation to occur in these cells. In line to altered G1/S-transition, we here additionally showed that subcellular abundance of proliferating cell nuclear antigen (PCNA), a marker for G1 and S phase sub-stages, was affected by B. besnoiti since infected cells showed increased nuclear PCNA levels when compared with that of control cells.
1000 Sacherschließung
lokal
lokal Coccidia
lokal Cattle Diseases/physiopathology [MeSH]
lokal Endothelial Cells/cytology [MeSH]
lokal Histone H3 S10
lokal Immunology and Host-Parasite Interactions - Original Paper
lokal M Phase Cell Cycle Checkpoints [MeSH]
lokal Coccidiosis/parasitology [MeSH]
lokal Cattle Diseases/parasitology [MeSH]
lokal Animals [MeSH]
lokal Apoptosis [MeSH]
lokal Endothelial Cells/parasitology [MeSH]
lokal G2 Phase Cell Cycle Checkpoints [MeSH]
lokal Coccidiosis/physiopathology [MeSH]
lokal Apicomplexan parasites
lokal Coccidiosis/veterinary [MeSH]
lokal Cell cycle arrest
lokal Cattle [MeSH]
lokal Sarcocystidae/physiology [MeSH]
1000 Liste der Beteiligten
  1. https://orcid.org/0000-0002-1360-2209|https://frl.publisso.de/adhoc/uri/TG9wZXotT3NvcmlvLCBTYXJh|https://frl.publisso.de/adhoc/uri/UGVydml6YWotT3J1cWFqLCBMZWFydGE=|https://frl.publisso.de/adhoc/uri/SGVyb2xkLCBTdXNhbm5l|https://frl.publisso.de/adhoc/uri/SGVybW9zaWxsYSwgQ2FybG9z|https://frl.publisso.de/adhoc/uri/VGF1YmVydCwgQW5qYQ==
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  1. Besnoitia besnoiti–driven endothelial host cell cycle alteration
1000 Objektart article
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1000 Erstellt am 2023-11-17T22:39:38.544+0100
1000 Erstellt von 322
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1000 Zuletzt bearbeitet 2023-12-01T09:47:58.505+0100
1000 Objekt bearb. Fri Dec 01 09:47:58 CET 2023
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