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Abstract/Summary
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<jats:sec><jats:title>Objective</jats:title><jats:p>Articular cartilage of the knee joint is avascular, exists under a low oxygen tension microenvironment, and does not self-heal when injured. Human infrapatellar fat pad-sourced mesenchymal stem cells (IFP-MSC) are an arthroscopically accessible source of mesenchymal stem cells (MSC) for the repair of articular cartilage defects. Human IFP-MSC exists physiologically under a low oxygen tension (i.e., 1–5%) microenvironment. Human bone marrow mesenchymal stem cells (BM-MSC) exist physiologically within a similar range of oxygen tension. A low oxygen tension of 2% spontaneously induced chondrogenesis in micromass pellets of human BM-MSC. However, this is yet to be demonstrated in human IFP-MSC or other adipose tissue-sourced MSC. In this study, we explored the potential of low oxygen tension at 2% to drive the <jats:italic>in vitro</jats:italic> chondrogenesis of IFP-MSC. We hypothesized that 2% O<jats:sub>2</jats:sub> will induce stable chondrogenesis in human IFP-MSC without the risk of undergoing endochondral ossification at ectopic sites of implantation.</jats:p></jats:sec><jats:sec><jats:title>Methods</jats:title><jats:p>Micromass pellets of human IFP-MSC were cultured under 2% O<jats:sub>2</jats:sub> or 21% O<jats:sub>2</jats:sub> (normal atmosphere O<jats:sub>2</jats:sub>) in the presence or absence of chondrogenic medium with transforming growth factor-β3 (TGFβ3) for 3 weeks. Following <jats:italic>in vitro</jats:italic> chondrogenesis, the resulting pellets were implanted in immunodeficient athymic nude mice for 3 weeks.</jats:p></jats:sec><jats:sec><jats:title>Results</jats:title><jats:p>A low oxygen tension of 2% was unable to induce chondrogenesis in human IFP-MSC. In contrast, chondrogenic medium with TGFβ3 induced <jats:italic>in vitro</jats:italic> chondrogenesis. All pellets were devoid of any evidence of undergoing endochondral ossification after subcutaneous implantation in athymic mice.</jats:p></jats:sec>
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1000
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Hinweis
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DeepGreen-ID: 0ef3c0c373fa4aeb90ff007453cbc36c ; metadata provieded by: DeepGreen (https://www.oa-deepgreen.de/api/v1/), LIVIVO search scope life sciences (http://z3950.zbmed.de:6210/livivo), Crossref Unified Resource API (https://api.crossref.org/swagger-ui/index.html), to.science.api (https://frl.publisso.de/), ZDB JSON-API (beta) (https://zeitschriftendatenbank.de/api/), lobid - Dateninfrastruktur für Bibliotheken (https://lobid.org/resources/search)
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