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1000 Titel
  • Validation of Microbiological Testing of Tissue Preparations with Different Incubation Temperatures
1000 Autor/in
  1. Herrlinger, Frithjof |
  2. Schulz, Tino |
  3. Pruß, Axel |
  4. Schulz, Eva |
1000 Erscheinungsjahr 2020
1000 Publikationstyp
  1. Artikel |
1000 Online veröffentlicht
  • 2020-12-16
1000 Erschienen in
1000 Quellenangabe
  • 48(1):23-31
1000 Copyrightjahr
  • 2020
1000 Embargo
  • 2021-12-23
1000 Lizenz
1000 Verlagsversion
  • https://doi.org/10.1159/000513646 |
  • https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7923897/ |
1000 Publikationsstatus
1000 Sprache der Publikation
1000 Abstract/Summary
  • Introduction!#!The European Pharmacopoeia (Ph. Eur.) provides principles for microbiological testing of tissue preparations. According to the Ph. Eur., tests should be performed at different temperatures for detection of aerobic bacteria and fungi (20-25°C) vs. anaerobic bacteria (30-35°C). Semiautomated systems using blood culture bottles are already widely used and they are adequate for growth detection. Resin-containing bottles and the addition of penicillinase permit testing of culture media containing antibiotics.!##!Materials and methods!#!At 3 temperatures (21, 30, and 35°C) cornea culture media with and without dextran (CM II and CM I) and thermal disinfected femoral head medium (FH) were spiked with the 6 reference strains recommended by the Ph. Eur. (additionally: !##!Results!#!Growth for all strains was detected with each medium at all 3 temperatures, except for !##!Conclusion!#!The requirements according to the Ph. Eur. for a successful method suitability test could be fulfilled for the semiautomated blood culture bottle system with the BACTEC
1000 Sacherschließung
lokal Tissue preparations
lokal Femoral head
lokal BACTEC
lokal Cornea
lokal Microbiological testing
lokal Temperature
lokal Research Article
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  1. https://frl.publisso.de/adhoc/uri/SGVycmxpbmdlciwgRnJpdGhqb2Y=|https://frl.publisso.de/adhoc/uri/U2NodWx6LCBUaW5v|https://frl.publisso.de/adhoc/uri/UHJ1w58sIEF4ZWw=|https://frl.publisso.de/adhoc/uri/U2NodWx6LCBFdmE=
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1000 Erstellt am 2024-04-11T13:10:15.646+0200
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