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1000
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Abstract/Summary
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<jats:p>Ozone (O<jats:sub>3</jats:sub>) stress severely affects the normal growth of grape (<jats:italic>Vitis vinifera</jats:italic> L.) leaves. Melatonin (MT) plays a significant role in plant response to various abiotic stresses, but its role in O<jats:sub>3</jats:sub> stress and related mechanisms are poorly understood. In order to understand the mechanism of MT in alleviate O<jats:sub>3</jats:sub> stress in grape leaves, we perform a transcriptome analyses of grapes leaves under O<jats:sub>3</jats:sub> stress with or without MT treatment. Transcriptome analysis showed that the processes of ethylene biosynthesis and signaling were clearly changed in “Cabernet Sauvignon” grapes under O<jats:sub>3</jats:sub> and MT treatment. O<jats:sub>3</jats:sub> stress induced the expression of genes related to ethylene biosynthesis and signal transduction, while MT treatment significantly inhibited the ethylene response mediated by O<jats:sub>3</jats:sub> stress. Further experiments showed that both MT and aminoethoxyvinylglycine (AVG, an inhibitor of ethylene biosynthesis) enhanced the photosynthetic and antioxidant capacities of grape leaves under O<jats:sub>3</jats:sub> stress, while ethephon inhibited those capacities. The combined treatment effect of MT and ethylene inhibitor was similar to that of MT alone. Exogenous MT reduced ethylene production in grape leaves under O<jats:sub>3</jats:sub> stress, while ethephon and ethylene inhibitors had little effect on the MT content of grape leaves after O<jats:sub>3</jats:sub> stress. However, overexpression of <jats:italic>VvACO2</jats:italic> (<jats:italic>1-aminocyclopropane-1-carboxylate oxidase2</jats:italic>) in grape leaves endogenously induced ethylene accumulation and aggravated O<jats:sub>3</jats:sub> stress. Overexpression of the MT synthesis gene <jats:italic>VvASMT1</jats:italic> (<jats:italic>acetylserotonin methyltransferase1</jats:italic>) in tobacco (<jats:italic>Nicotiana tabacum</jats:italic> L.) alleviated O<jats:sub>3</jats:sub> stress and reduced ethylene biosynthesis after O<jats:sub>3</jats:sub> stress. In summary, MT can alleviate O<jats:sub>3</jats:sub> stress in grape leaves by inhibiting ethylene biosynthesis.</jats:p>
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1000
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Hinweis
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DeepGreen-ID: 30bb4c8b2d74454e8083c789a25749b4 ; metadata provieded by: DeepGreen (https://www.oa-deepgreen.de/api/v1/), LIVIVO search scope life sciences (http://z3950.zbmed.de:6210/livivo), Crossref Unified Resource API (https://api.crossref.org/swagger-ui/index.html), to.science.api (https://frl.publisso.de/), ZDB JSON-API (beta) (https://zeitschriftendatenbank.de/api/), lobid - Dateninfrastruktur für Bibliotheken (https://lobid.org/resources/search)
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