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1000 Titel
  • Comparison of Multiscale Imaging Methods for Brain Research
1000 Autor/in
  1. Tröger, Jessica |
  2. Hoischen, Christian |
  3. Perner, Birgit |
  4. Monajembashi, Shamci |
  5. Barbotin, Aurélien |
  6. Löschberger, Anna |
  7. Eggeling, Christian |
  8. Kessels, Michael M. |
  9. Qualmann, Britta |
  10. Hemmerich, Peter |
1000 Erscheinungsjahr 2020
1000 LeibnizOpen
1000 Publikationstyp
  1. Artikel |
1000 Online veröffentlicht
  • 2020-06-01
1000 Erschienen in
1000 Quellenangabe
  • 9(6):1377
1000 FRL-Sammlung
1000 Copyrightjahr
  • 2020
1000 Lizenz
1000 Verlagsversion
  • https://doi.org/10.3390/cells9061377 |
  • https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7349602/ |
1000 Publikationsstatus
1000 Begutachtungsstatus
1000 Sprache der Publikation
1000 Abstract/Summary
  • A major challenge in neuroscience is how to study structural alterations in the brain. Even small changes in synaptic composition could have severe outcomes for body functions. Many neuropathological diseases are attributable to disorganization of particular synaptic proteins. Yet, to detect and comprehensively describe and evaluate such often rather subtle deviations from the normal physiological status in a detailed and quantitative manner is very challenging. Here, we have compared side-by-side several commercially available light microscopes for their suitability in visualizing synaptic components in larger parts of the brain at low resolution, at extended resolution as well as at super-resolution. Microscopic technologies included stereo, widefield, deconvolution, confocal, and super-resolution set-ups. We also analyzed the impact of adaptive optics, a motorized objective correction collar and CUDA graphics card technology on imaging quality and acquisition speed. Our observations evaluate a basic set of techniques, which allow for multi-color brain imaging from centimeter to nanometer scales. The comparative multi-modal strategy we established can be used as a guide for researchers to select the most appropriate light microscopy method in addressing specific questions in brain research, and we also give insights into recent developments such as optical aberration corrections.
1000 Sacherschließung
lokal Imaging, Three-Dimensional
lokal Synapses/physiology [MeSH]
lokal Neurons/cytology [MeSH]
lokal Rats [MeSH]
lokal Research [MeSH]
lokal Animals [MeSH]
lokal Mice
lokal Mice [MeSH]
lokal Brain/anatomy
lokal Male [MeSH]
lokal Single-Cell Analysis
lokal Male
lokal Rats
lokal Research
lokal Single-Cell Analysis [MeSH]
lokal Synapses/physiology
lokal Microscopy, Confocal
lokal Animals
lokal Imaging, Three-Dimensional [MeSH]
lokal Microscopy, Confocal [MeSH]
lokal Neurons/cytology
1000 Fächerklassifikation (DDC)
1000 Liste der Beteiligten
  1. https://frl.publisso.de/adhoc/uri/VHLDtmdlciwgSmVzc2ljYQ==|https://frl.publisso.de/adhoc/uri/SG9pc2NoZW4sIENocmlzdGlhbg==|https://frl.publisso.de/adhoc/uri/UGVybmVyLCBCaXJnaXQ=|https://frl.publisso.de/adhoc/uri/TW9uYWplbWJhc2hpLCBTaGFtY2k=|https://frl.publisso.de/adhoc/uri/QmFyYm90aW4sIEF1csOpbGllbg==|https://frl.publisso.de/adhoc/uri/TMO2c2NoYmVyZ2VyLCBBbm5h|https://orcid.org/0000-0002-3698-5599|https://frl.publisso.de/adhoc/uri/S2Vzc2VscywgTWljaGFlbCBNLg==|https://frl.publisso.de/adhoc/uri/UXVhbG1hbm4sIEJyaXR0YQ==|https://orcid.org/0000-0003-0719-6985
1000 Label
1000 Fördernummer
  1. -
1000 Förderprogramm
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1000 Dateien
  1. Comparison of Multiscale Imaging Methods for Brain Research
1000 Objektart article
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1000 @id frl:6475675.rdf
1000 Erstellt am 2024-05-08T09:52:00.585+0200
1000 Erstellt von 336
1000 beschreibt frl:6475675
1000 Bearbeitet von 317
1000 Zuletzt bearbeitet 2024-05-27T11:09:45.775+0200
1000 Objekt bearb. Mon May 27 11:09:26 CEST 2024
1000 Vgl. frl:6475675
1000 Oai Id
  1. oai:frl.publisso.de:frl:6475675 |
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