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1000
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Abstract/Summary
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<jats:p>The betaproteobacterial genus <i>Aromatoleum</i> comprises facultative denitrifiers specialized in the anaerobic degradation of recalcitrant organic compounds (aromatic and terpenoid). This study reports on the complete and manually annotated genomes of <i>Ar. petrolei</i> ToN1<sup>T</sup> (5.41 Mbp) and <i>Ar. bremense</i> PbN1<sup>T</sup> (4.38 Mbp), which cover the phylogenetic breadth of the genus <i>Aromatoleum</i> together with previously genome sequenced <i>Ar. aromaticum</i> EbN1<sup>T</sup> [Rabus et al., Arch Microbiol. 2005 Jan;183(1):27–36]. The gene clusters for the anaerobic degradation of aromatic and terpenoid (strain ToN1<sup>T</sup> only) compounds are scattered across the genomes of strains ToN1<sup>T</sup> and PbN1<sup>T</sup>. The richness in mobile genetic elements is shared with other <i>Aromatoleum</i> spp., substantiating that horizontal gene transfer should have been a major driver in shaping the genomes of this genus. The composite catabolic network of strains ToN1<sup>T</sup> and PbN1<sup>T</sup> comprises 88 proteins, the coding genes of which occupy 86.1 and 76.4 kbp (1.59 and 1.75%) of the respective genome. The strain-specific gene clusters for anaerobic degradation of ethyl-/propylbenzene (strain PbN1<sup>T</sup>) and toluene/monoterpenes (strain ToN1<sup>T</sup>) share high similarity with their counterparts in <i>Ar. aromaticum</i> strains EbN1<sup>T</sup> and pCyN1, respectively. Glucose is degraded via the ED-pathway in strain ToN1<sup>T</sup>, while gluconeogenesis proceeds via the reverse EMP-pathway in strains ToN1<sup>T</sup>, PbN1<sup>T</sup>, and EbN1<sup>T</sup>. The diazotrophic, endophytic lifestyle of closest related genus <i>Azoarcus</i> is known to be associated with nitrogenase and type-6 secretion system (T6SS). By contrast, strains ToN1<sup>T</sup>, PbN1<sup>T</sup>, and EbN1<sup>T</sup> lack <i>nif</i> genes for nitrogenase (including cofactor synthesis and enzyme maturation). Moreover, strains PbN1<sup>T</sup> and EbN1<sup>T</sup> do not possess <i>tss</i> genes for T6SS, while strain ToN1<sup>T</sup> does and facultative endophytic “<i>Aromatoleum</i>” sp. CIB is known to even have both. These findings underpin the functional heterogeneity among <i>Aromatoleum</i> members, correlating with the high plasticity of their genomes. </jats:p>
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1000
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Hinweis
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