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Abstract/Summary
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<jats:sec><jats:title>Objective</jats:title><jats:p>Understanding the characteristics of tumor suppressor genes (TSGs) is of great significance for the development of new targeted treatment strategies for non-small cell lung cancer (NSCLC). Therefore, this present article is to explore the underlying molecular mechanism of ZFN24 inhibiting the development of NSCLC.</jats:p></jats:sec><jats:sec><jats:title>Methods</jats:title><jats:p>We performed RT-PCR and Western blotting for evaluating associated RNA and protein expression. CCK8, colony forming and sphere-forming assays were used to evaluate the proliferation and stemness of NSCLC cells. NSCLC cell senescence was examined by <jats:italic>β</jats:italic>-galactosidase staining assay. Luciferase assay was performed to evaluate <jats:italic>β</jats:italic>-catenin transcriptional activity. The effect of ZNF24 on NSCLC cells <jats:italic>in vivo</jats:italic> was evaluated by the xenograft tumor experiment.</jats:p></jats:sec><jats:sec><jats:title>Results</jats:title><jats:p>Ectopic expression of ZNF24 significantly inhibited cell viability, colony forming ability, and stemness of NSCLC cells. WNT signaling pathway was inhibited by ZNF24 resulting in NSCLC cell senescence. <jats:italic>β</jats:italic>-catenin transcriptional activity was significantly inhibited by ZNF24 (P &lt; 0.05). Ectopic expression of ZNF24 significantly inhibited xenotransplant tumors growth <jats:italic>in vivo</jats:italic> (P &lt; 0.05).</jats:p></jats:sec><jats:sec><jats:title>Conclusion</jats:title><jats:p>ZNF24 could notably inhibit the development of NSCLC by inhibiting the WNT signaling pathway.</jats:p></jats:sec>
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Hinweis
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DeepGreen-ID: afb6dc6f5c5f48c6a52e6e6df66f5cbd ; metadata provieded by: DeepGreen (https://www.oa-deepgreen.de/api/v1/), LIVIVO search scope life sciences (http://z3950.zbmed.de:6210/livivo), Crossref Unified Resource API (https://api.crossref.org/swagger-ui/index.html), to.science.api (https://frl.publisso.de/), ZDB JSON-API (beta) (https://zeitschriftendatenbank.de/api/), lobid - Dateninfrastruktur für Bibliotheken (https://lobid.org/resources/search)
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