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1000
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Abstract/Summary
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<jats:p>Bacteria use K<jats:sup>+</jats:sup>-uptake transporters differentially for adaptation in varying growth conditions. In <jats:italic>Mycobacterium tuberculosis</jats:italic>, two K<jats:sup>+</jats:sup>-uptake systems, the Trk comprising the CeoB and CeoC proteins and the Kdp consisting of the two-component system (TCS), KdpDE and KdpFABC, have been characterized, but their selective utilization during bacterial growth has not been completely explored. In the current study, the roles of the <jats:italic>M. tuberculosis</jats:italic> KdpDE regulatory system alone and in association with the Trk transporters in bacterial growth were investigated by evaluating the growth of <jats:italic>M. tuberculosis</jats:italic> KdpDE-deletion and KdpDE/Trk (KT)-double knockout mutant strains in planktonic culture under standard growth conditions. The KT-double knockout mutant strain was first constructed using homologous recombination procedures and was evaluated together with the KdpDE-deletion mutant and the wild-type (WT) strains with respect to their rates of growth, K<jats:sup>+</jats:sup>-uptake efficiencies, and K<jats:sup>+</jats:sup>-transporter gene expression during planktonic growth. During growth at optimal K<jats:sup>+</jats:sup> concentrations and pH levels, selective deletion of the TCS KdpDE (KdpDE-deletion mutant) led to attenuation of bacterial growth and an increase in bacterial K<jats:sup>+</jats:sup>-uptake efficiency, as well as dysregulated expression of the <jats:italic>kdpFABC</jats:italic> and <jats:italic>trk</jats:italic> genes. Deletion of both the KdpDE and the Trk systems (KT-double knockout) also led to severely attenuated bacterial growth, as well as an increase in bacterial K<jats:sup>+</jats:sup>-uptake efficiency. These results demonstrate that the KdpDE regulatory system plays a key role during bacterial growth by regulating K<jats:sup>+</jats:sup> uptake <jats:italic>via</jats:italic> modulation of the expression and activities of both the KdpFABC and Trk systems and is important for bacterial growth possibly by preventing cytoplasmic K<jats:sup>+</jats:sup> overload.</jats:p>
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1000
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Hinweis
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DeepGreen-ID: 670861af5de64c8aba3494fdf90591bf ; metadata provieded by: DeepGreen (https://www.oa-deepgreen.de/api/v1/), LIVIVO search scope life sciences (http://z3950.zbmed.de:6210/livivo), Crossref Unified Resource API (https://api.crossref.org/swagger-ui/index.html), to.science.api (https://frl.publisso.de/), ZDB JSON-API (beta) (https://zeitschriftendatenbank.de/api/), lobid - Dateninfrastruktur für Bibliotheken (https://lobid.org/resources/search)
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