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1000
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Abstract/Summary
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<jats:p>As the most abundant and reversible chemical modification in eukaryotic mRNA, the epitranscriptomic mark <jats:italic>N</jats:italic><jats:sup>6</jats:sup>-methyladenine (m<jats:sup>6</jats:sup>A) regulates plant development and stress response. We have previously characterized that ALKBH10B is an Arabidopsis mRNA m<jats:sup>6</jats:sup>A demethylase and regulates floral transition. However, it is unclear whether ALKBH10B plays a role in abiotic stress response. Here, we found that the expression of <jats:italic>ALKBH10B</jats:italic> is increased in response to abscisic acid (ABA), osmotic, and salt stress. The <jats:italic>alkbh10b</jats:italic> mutants showed hypersensitive to ABA, osmotic, and salt stress during seed germination. Transcriptome analysis revealed that the expression of several ABA response genes is upregulated in <jats:italic>alkbh10b-1</jats:italic> than that of wild type, indicating ALKBH10B negatively affects the ABA signaling. Furthermore, m<jats:sup>6</jats:sup>A sequencing showed that ABA signaling genes, including <jats:italic>PYR1</jats:italic>, <jats:italic>PYL7</jats:italic>, <jats:italic>PYL9</jats:italic>, <jats:italic>ABI1</jats:italic>, and <jats:italic>SnRK2.2</jats:italic> are m<jats:sup>6</jats:sup>A hypermethylated in <jats:italic>alkbh10b-1</jats:italic> after ABA treatment. Taken together, our work demonstrated that ALKBH10B negatively modulates ABA response during seed germination in Arabidopsis.</jats:p>
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1000
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Hinweis
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DeepGreen-ID: 88d6d3e1dd33437c8d626b71ba8bf040 ; metadata provieded by: DeepGreen (https://www.oa-deepgreen.de/api/v1/), LIVIVO search scope life sciences (http://z3950.zbmed.de:6210/livivo), Crossref Unified Resource API (https://api.crossref.org/swagger-ui/index.html), to.science.api (https://frl.publisso.de/), ZDB JSON-API (beta) (https://zeitschriftendatenbank.de/api/), lobid - Dateninfrastruktur für Bibliotheken (https://lobid.org/resources/search)
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