|
1000
|
Abstract/Summary
|
-
<jats:p>The G protein-coupled receptor (GPR) family critically regulates development and homeostasis of multiple organs. As a member of the GPR adhesion family, Gpr125 (Adgra3) modulates Wnt/PCP signaling and convergent extension in developing zebrafish, but whether it is essential for cochlear development in mammals is unknown. Here, we examined the <jats:italic>Gpr125</jats:italic><jats:sup><jats:italic>lacZ/+</jats:italic></jats:sup> knock-in mice and show that Gpr125 is dynamically expressed in the developing and mature cochleae. From embryonic day (E) 15.5 to postnatal day (P) 30, Gpr125-β-Gal is consistently expressed in the lesser epithelial ridge and its presumed progenies, the supporting cell subtypes Claudius cells and Hensen’s cells. In contrast, Gpr125-β-Gal is expressed transiently in outer hair cells, epithelial cells in the lateral cochlear wall, interdental cells, and spiral ganglion neurons in the late embryonic and early postnatal cochlea. <jats:italic>In situ</jats:italic> hybridization for <jats:italic>Gpr125</jats:italic> mRNA confirmed <jats:italic>Gpr125</jats:italic> expression and validated loss of expression in <jats:italic>Gpr125</jats:italic><jats:italic><jats:sup><jats:italic>lacZ/lacZ</jats:italic></jats:sup></jats:italic> cochleae. Lastly, <jats:italic>Gpr125</jats:italic><jats:sup><jats:italic>lacZ/+</jats:italic></jats:sup> and <jats:italic>Gpr125</jats:italic><jats:italic><jats:sup><jats:italic>lacZ/</jats:italic><jats:italic>lacZ</jats:italic></jats:sup></jats:italic> cochleae displayed no detectable loss or disorganization of either sensory or non-sensory cells in the embryonic and postnatal ages and exhibited normal auditory physiology. Together, our study reveals that Gpr125 is dynamically expressed in multiple cell types in the developing and mature cochlea and is dispensable for cochlear development and hearing.</jats:p>
|
|
1000
|
Hinweis
|
-
DeepGreen-ID: 8fc8f167f52e407c829c3d1b730f24e2 ; metadata provieded by: DeepGreen (https://www.oa-deepgreen.de/api/v1/), LIVIVO search scope life sciences (http://z3950.zbmed.de:6210/livivo), Crossref Unified Resource API (https://api.crossref.org/swagger-ui/index.html), to.science.api (https://frl.publisso.de/), ZDB JSON-API (beta) (https://zeitschriftendatenbank.de/api/), lobid - Dateninfrastruktur für Bibliotheken (https://lobid.org/resources/search)
|
