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1000
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Abstract/Summary
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<jats:p>The conversion of cytidines to uridines (C-to-U) at specific sites in mitochondrial and plastid transcripts is a post-transcriptional processing event that is important to the expression of organellar genes. Pentatricopeptide repeat (PPR) proteins are involved in this process. In this study, we report the function of a previously uncharacterized PPR-DYW protein, Empty pericarp17 (EMP17), in the C-to-U editing and kernel development in maize. EMP17 is targeted to mitochondria. The loss-function of EMP17 arrests maize kernel development, abolishes the editing at <jats:italic>ccmF</jats:italic><jats:sub><jats:italic>C</jats:italic></jats:sub>-799 and <jats:italic>nad2</jats:italic>-677 sites, and reduces the editing at <jats:italic>ccmF</jats:italic><jats:sub><jats:italic>C</jats:italic></jats:sub>-906 and -966 sites. The absence of editing causes amino acid residue changes in CcmF<jats:sub>C</jats:sub>-267 (Ser to Pro) and Nad2-226 (Phe to Ser), respectively. As CcmF<jats:sub>C</jats:sub> functions in cytochrome <jats:italic>c</jats:italic> (Cyt<jats:italic>c</jats:italic>) maturation, the amount of Cyt<jats:italic>c</jats:italic> and Cyt<jats:italic>c</jats:italic><jats:sub>1</jats:sub> protein is drastically reduced in <jats:italic>emp17</jats:italic>, suggesting that the CcmF<jats:sub>C</jats:sub>-267 (Ser to Pro) change impairs the CcmF<jats:sub>C</jats:sub> function. As a result, the assembly of complex III is strikingly decreased in <jats:italic>emp17</jats:italic>. In contrast, the assembly of complex I appears less affected, suggesting that the Nad2-226 (Phe to Ser) change may have less impact on Nad2 function. Together, these results indicate that EMP17 is required for the C-to-U editing at several sites in mitochondrial transcripts, complex III biogenesis, and seed development in maize.</jats:p>
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1000
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Hinweis
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DeepGreen-ID: e39cb1fb04a64d969b5c4d594d5ea0cc ; metadata provieded by: DeepGreen (https://www.oa-deepgreen.de/api/v1/), LIVIVO search scope life sciences (http://z3950.zbmed.de:6210/livivo), Crossref Unified Resource API (https://api.crossref.org/swagger-ui/index.html), to.science.api (https://frl.publisso.de/), ZDB JSON-API (beta) (https://zeitschriftendatenbank.de/api/), lobid - Dateninfrastruktur für Bibliotheken (https://lobid.org/resources/search)
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