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1000
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Abstract/Summary
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Among venomous animals, toxic secretions have evolved as biochemical weapons associated with various highly specialized delivery systems on many occasions. Despite extensive research, there is still limited knowledge of the functional biology of most animal toxins, including their venom production and storage, as well as the morphological structures within sophisticated venom producing tissues that might underpin venom modulation. Here we report on the spatial exploration of a snake venom gland system by matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI-MSI), in combination with standard proteotranscriptomic approaches, to enable in situ toxin mapping in spatial intensity maps across a venom gland sourced from the Egyptian cobra (Naja haje). MALDI-MSI toxin visualization on the elapid venom gland reveals high spatial heterogeneity of different toxin classes at the proteoform level, which may be the result of physiological constraints on venom production and/or storage that reflects the potential for venom modulation under diverse stimuli.
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1000
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Hinweis
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This is the preprint version of this publication: "Benjamin-Florian Hempel, Maik Damm, Daniel Petras, Taline D. Kazandjian, Claudia A. Szentiks, Guido Fritsch, Grit Nebrich, Nicholas R. Casewell, Oliver Klein, and Roderich D. Süssmuth. Journal of Proteome Research 2023 22 (1), 26-35. DOI: 10.1021/acs.jproteome.2c00424"
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