ERFVII-controlled hypoxia responses are in part facilitated by MEDIATOR SUBUNIT 25 in Arabidopsis thaliana

  1. Schippers, Jozefus ORCID logo
  2. von Bongartz, Kira
  3. Laritzki, Lisa
  4. Frohn, Stephanie
  5. Frings, Stephanie ORCID logo
  6. , Tilo Renziehausen ORCID logo
  7. Augstein, Frauke ORCID logo
  8. Winkels, Katharina ORCID logo
  9. Sprangers, Katrien
  10. Sasidharan, Rashmi ORCID logo
  11. Vertommen, Didier ORCID logo
  12. Van Breusegem, Frank ORCID logo
  13. Hartman, Sjon ORCID logo
  14. Beemster, Gerrit ORCID logo
  15. Mhamdi, Amna ORCID logo
  16. van Dongen, Joost ORCID logo
  17. Schmidt-Schippers, Romy ORCID logo

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1000 Titel
  • ERFVII-controlled hypoxia responses are in part facilitated by MEDIATOR SUBUNIT 25 in Arabidopsis thaliana
1000 Autor/in
  1. Schippers, Jozefus |
  2. von Bongartz, Kira |
  3. Laritzki, Lisa |
  4. Frohn, Stephanie |
  5. Frings, Stephanie |
  6. , Tilo Renziehausen |
  7. Augstein, Frauke |
  8. Winkels, Katharina |
  9. Sprangers, Katrien |
  10. Sasidharan, Rashmi |
  11. Vertommen, Didier |
  12. Van Breusegem, Frank |
  13. Hartman, Sjon |
  14. Beemster, Gerrit |
  15. Mhamdi, Amna |
  16. van Dongen, Joost |
  17. Schmidt-Schippers, Romy |
1000 Erscheinungsjahr 2024
1000 LeibnizOpen
1000 Publikationstyp
  1. Artikel |
1000 Online veröffentlicht
  • 2024-09-11
1000 Erschienen in
1000 Quellenangabe
  • 120(2):748-768
1000 FRL-Sammlung
1000 Copyrightjahr
  • 2024
1000 Lizenz
1000 Verlagsversion
  • https://dx.doi.org/10.1111/tpj.17018 |
1000 Ergänzendes Material
  • https://onlinelibrary.wiley.com/doi/10.1111/tpj.17018#support-information-section |
1000 Publikationsstatus
1000 Begutachtungsstatus
1000 Sprache der Publikation
1000 Abstract/Summary
  • Flooding impairs plant growth through oxygen deprivation, which activates plant survival and acclimation responses. Transcriptional responses to low oxygen are generally associated with the activation of group VII ETHYLENE-RESPONSE FACTOR (ERFVII) transcription factors. However, the exact mechanisms and molecular components by which ERFVII factors initiate gene expression are not fully elucidated. Here, we show that the ERFVII factors RELATED TO APETALA 2.2 (RAP2.2) and RAP2.12 cooperate with the Mediator complex subunit AtMED25 to coordinate gene expression under hypoxia in Arabidopsis thaliana. Respective med25 knock-out mutants display reduced low-oxygen stress tolerance. AtMED25 physically associates with a distinct set of hypoxia core genes and its loss partially impairs transcription under hypoxia due to decreased RNA polymerase II recruitment. Association of AtMED25 with target genes requires the presence of ERFVII transcription factors. Next to ERFVII protein stabilisation, also the composition of the Mediator complex including AtMED25 is potentially affected by hypoxia stress as shown by protein-complex pulldown assays. The dynamic response of the Mediator complex to hypoxia is furthermore supported by the fact that two subunits, AtMED8 and AtMED16, are not involved in the establishment of hypoxia tolerance, whilst both act in coordination with AtMED25 under other environmental conditions. We furthermore show that AtMED25 function under hypoxia is independent of ethylene signalling. Finally, functional conservation at the molecular level was found for the MED25-ERFVII module between A. thaliana and the monocot species Oryza sativa, pointing to a potentially universal role of MED25 in coordinating ERFVII-dependent transcript responses to hypoxia in plants.
1000 Sacherschließung
lokal ERFVII factors
lokal flooding
lokal Mediator complex
lokal hypoxia
lokal transcription
1000 Fächerklassifikation (DDC)
1000 Liste der Beteiligten
  1. https://orcid.org/0000-0001-7934-126X|https://frl.publisso.de/adhoc/uri/dm9uIEJvbmdhcnR6LCBLaXJh|https://frl.publisso.de/adhoc/uri/TGFyaXR6a2ksIExpc2E=|https://frl.publisso.de/adhoc/uri/RnJvaG4sIFN0ZXBoYW5pZQ==|https://orcid.org/0000-0003-3024-966X|https://orcid.org/0009-0004-5502-4234|https://orcid.org/0000-0003-3625-9703|https://orcid.org/0000-0003-1276-6970|https://frl.publisso.de/adhoc/uri/U3ByYW5nZXJzLCBLYXRyaWVu|https://orcid.org/0000-0002-6940-0657|https://orcid.org/0000-0001-7648-8282|https://orcid.org/0000-0002-3147-0860|https://orcid.org/0000-0002-6709-6436|https://orcid.org/0000-0001-6014-053X|https://orcid.org/0000-0001-9959-1362|https://orcid.org/0000-0001-7944-9289|https://orcid.org/0000-0002-3395-0673
1000 Label
1000 Förderer
  1. Deutsche Forschungsgemeinschaft |
  2. Fonds Wetenschappelijk Onderzoek |
  3. Projekt DEAL |
1000 Fördernummer
  1. SCHM3345/2-1;StUpPD_332-18
  2. 30829584;G007723N
  3. -
1000 Förderprogramm
  1. -
  2. -
  3. Open Access funding
1000 Dateien
1000 Förderung
  1. 1000 joinedFunding-child
    1000 Förderer Deutsche Forschungsgemeinschaft |
    1000 Förderprogramm -
    1000 Fördernummer SCHM3345/2-1;StUpPD_332-18
  2. 1000 joinedFunding-child
    1000 Förderer Fonds Wetenschappelijk Onderzoek |
    1000 Förderprogramm -
    1000 Fördernummer 30829584;G007723N
  3. 1000 joinedFunding-child
    1000 Förderer Projekt DEAL |
    1000 Förderprogramm Open Access funding
    1000 Fördernummer -
1000 Objektart article
1000 Beschrieben durch
1000 @id frl:6488887.rdf
1000 Erstellt am 2024-11-04T11:41:49.189+0100
1000 Erstellt von 325
1000 beschreibt frl:6488887
1000 Bearbeitet von 317
1000 Zuletzt bearbeitet 2025-08-06T15:14:17.690+0200
1000 Objekt bearb. Mon Nov 04 14:00:27 CET 2024
1000 Vgl. frl:6488887
1000 Oai Id
  1. oai:frl.publisso.de:frl:6488887 |
1000 Sichtbarkeit Metadaten public
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