The auxin efflux carrier PIN1a regulates vascular patterning in cereal roots

  1. Fusi, Riccardo ORCID logo
  2. Milner, Sara Giulia ORCID logo
  3. Rosignoli, Serena ORCID logo
  4. Bovina, Riccardo
  5. Vieira Teixeira, Cristovão ORCID logo
  6. Lou, Haoyu ORCID logo
  7. Atkinson, Brian ORCID logo
  8. Borkar, Aditi Narendra ORCID logo
  9. York, Larry ORCID logo
  10. Jones, Dylan ORCID logo
  11. Sturrock, Craig ORCID logo
  12. Stein, Nils ORCID logo
  13. Mascher, Martin ORCID logo
  14. TUBEROSA, ROBERTO ORCID logo
  15. O'Connor, Devin ORCID logo
  16. Bennett, Malcolm ORCID logo
  17. Bishopp, Anthony ORCID logo
  18. SALVI, SILVIO ORCID logo
  19. Bhosale, Rahul ORCID logo

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WeightNameValue
1000 Titel
  • The auxin efflux carrier PIN1a regulates vascular patterning in cereal roots
1000 Autor/in
  1. Fusi, Riccardo |
  2. Milner, Sara Giulia |
  3. Rosignoli, Serena |
  4. Bovina, Riccardo |
  5. Vieira Teixeira, Cristovão |
  6. Lou, Haoyu |
  7. Atkinson, Brian |
  8. Borkar, Aditi Narendra |
  9. York, Larry |
  10. Jones, Dylan |
  11. Sturrock, Craig |
  12. Stein, Nils |
  13. Mascher, Martin |
  14. TUBEROSA, ROBERTO |
  15. O'Connor, Devin |
  16. Bennett, Malcolm |
  17. Bishopp, Anthony |
  18. SALVI, SILVIO |
  19. Bhosale, Rahul |
1000 Erscheinungsjahr 2024
1000 LeibnizOpen
1000 Publikationstyp
  1. Artikel |
1000 Online veröffentlicht
  • 2024-04-26
1000 Erschienen in
1000 Quellenangabe
  • 244(1):104-115
1000 FRL-Sammlung
1000 Copyrightjahr
  • 2024
1000 Lizenz
1000 Verlagsversion
  • https://dx.doi.org/10.1111/nph.19777 |
1000 Publikationsstatus
1000 Begutachtungsstatus
1000 Sprache der Publikation
1000 Abstract/Summary
  • Barley (Hordeum vulgare) is an important global cereal crop and a model in genetic studies. Despite advances in characterising barley genomic resources, few mutant studies have identified genes controlling root architecture and anatomy, which plays a critical role in capturing soil resources. Our phenotypic screening of a TILLING mutant collection identified line TM5992 exhibiting a short-root phenotype compared with wild-type (WT) Morex background. Outcrossing TM5992 with barley variety Proctor and subsequent SNP array-based bulk segregant analysis, fine mapped the mutation to a cM scale. Exome sequencing pinpointed a mutation in the candidate gene HvPIN1a, further confirming this by analysing independent mutant alleles. Detailed analysis of root growth and anatomy in Hvpin1a mutant alleles exhibited a slower growth rate, shorter apical meristem and striking vascular patterning defects compared to WT. Expression and mutant analyses of PIN1 members in the closely related cereal brachypodium (Brachypodium distachyon) revealed that BdPIN1a and BdPIN1b were redundantly expressed in root vascular tissues but only Bdpin1a mutant allele displayed root vascular defects similar to Hvpin1a. We conclude that barley PIN1 genes have sub-functionalised in cereals, compared to Arabidopsis (Arabidopsis thaliana), where PIN1a sequences control root vascular patterning.
1000 Sacherschließung
lokal auxin efflux carrier
lokal PIN1
lokal root
lokal barley
lokal vascular development
1000 Fächerklassifikation (DDC)
1000 Liste der Beteiligten
  1. https://orcid.org/0000-0001-9785-0244|https://orcid.org/0000-0002-1600-5979|https://orcid.org/0000-0002-4319-8761|https://frl.publisso.de/adhoc/uri/Qm92aW5hLCBSaWNjYXJkbw==|https://orcid.org/0000-0003-3984-5513|https://orcid.org/0000-0001-6422-7157|https://orcid.org/0000-0001-8791-1613|https://orcid.org/0000-0003-4361-6561|https://orcid.org/0000-0002-1995-9479|https://orcid.org/0000-0001-8366-1995|https://orcid.org/0000-0002-5333-8502|https://orcid.org/0000-0003-3011-8731|https://orcid.org/0000-0001-6373-6013|https://orcid.org/0000-0001-9143-9569|https://orcid.org/0000-0003-4071-8626|https://orcid.org/0000-0003-0475-390X|https://orcid.org/0000-0003-2962-9542|https://orcid.org/0000-0002-0338-8894|https://orcid.org/0000-0001-6515-4922
1000 Label
1000 Förderer
  1. European Research Council |
  2. Biotechnology and Biological Sciences Research Council |
1000 Fördernummer
  1. 294729
  2. BB/S011102/1;BB/X014843/1;BB/X018806/1
1000 Förderprogramm
  1. Futureroots
  2. -
1000 Dateien
1000 Förderung
  1. 1000 joinedFunding-child
    1000 Förderer European Research Council |
    1000 Förderprogramm Futureroots
    1000 Fördernummer 294729
  2. 1000 joinedFunding-child
    1000 Förderer Biotechnology and Biological Sciences Research Council |
    1000 Förderprogramm -
    1000 Fördernummer BB/S011102/1;BB/X014843/1;BB/X018806/1
1000 Objektart article
1000 Beschrieben durch
1000 @id frl:6489106.rdf
1000 Erstellt am 2024-11-21T13:22:37.100+0100
1000 Erstellt von 325
1000 beschreibt frl:6489106
1000 Bearbeitet von 317
1000 Zuletzt bearbeitet 2025-02-24T12:48:21.903+0100
1000 Objekt bearb. Mon Feb 24 12:48:07 CET 2025
1000 Vgl. frl:6489106
1000 Oai Id
  1. oai:frl.publisso.de:frl:6489106 |
1000 Sichtbarkeit Metadaten public
1000 Sichtbarkeit Daten public
1000 Gegenstand von

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