HOMEOBOX2, the paralog of SIX-ROWED SPIKE1/HOMEOBOX1, is dispensable for barley spikelet development

  1. Thirulogachandar, Venkatasubbbu ORCID logo
  2. , Geetha Govind ORCID logo
  3. Hensel, Götz ORCID logo
  4. Kale, Sandip ORCID logo
  5. Kuhlmann, Markus ORCID logo
  6. Eschen-Lippold, Lennart ORCID logo
  7. Rutten, Twan ORCID logo
  8. Koppolu, Ravi ORCID logo
  9. Rajaraman, Jeyaraman ORCID logo
  10. Palakolanu, Sudhakar Reddy ORCID logo
  11. Seiler, Christiane ORCID logo
  12. Sakuma, Shun ORCID logo
  13. Jayakodi, Murukarthick ORCID logo
  14. Lee, Justin ORCID logo
  15. Kumlehn, Jochen ORCID logo
  16. Komatsuda, Takao ORCID logo
  17. Schnurbusch, Thorsten ORCID logo
  18. Sreenivasulu, Nese ORCID logo

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WeightNameValue
1000 Titel
  • HOMEOBOX2, the paralog of SIX-ROWED SPIKE1/HOMEOBOX1, is dispensable for barley spikelet development
1000 Autor/in
  1. Thirulogachandar, Venkatasubbbu |
  2. , Geetha Govind |
  3. Hensel, Götz |
  4. Kale, Sandip |
  5. Kuhlmann, Markus |
  6. Eschen-Lippold, Lennart |
  7. Rutten, Twan |
  8. Koppolu, Ravi |
  9. Rajaraman, Jeyaraman |
  10. Palakolanu, Sudhakar Reddy |
  11. Seiler, Christiane |
  12. Sakuma, Shun |
  13. Jayakodi, Murukarthick |
  14. Lee, Justin |
  15. Kumlehn, Jochen |
  16. Komatsuda, Takao |
  17. Schnurbusch, Thorsten |
  18. Sreenivasulu, Nese |
1000 Erscheinungsjahr 2024
1000 LeibnizOpen
1000 Publikationstyp
  1. Artikel |
1000 Online veröffentlicht
  • 2024-02-15
1000 Erschienen in
1000 Quellenangabe
  • 75(10):2900-2916
1000 FRL-Sammlung
1000 Copyrightjahr
  • 2024
1000 Lizenz
1000 Verlagsversion
  • https://dx.doi.org/10.1093/jxb/erae044 |
  • https://pmc.ncbi.nlm.nih.gov/articles/PMC11358255/ |
1000 Publikationsstatus
1000 Begutachtungsstatus
1000 Sprache der Publikation
1000 Abstract/Summary
  • The HD-ZIP class I transcription factor, HvHOX1 (Homeobox 1) or VRS1 (Vulgare Row-type Spike 1 or Six-rowed Spike 1), regulates lateral spikelet fertility in barley (Hordeum vulgare L.). It was shown that HvHOX1 has a high expression only in lateral spikelets, while its paralog HvHOX2 was found to be expressed in different plant organs. Yet, the mechanistic function of HvHOX1 and HvHOX2 during spikelet development is still fragmentary. Here, we show that compared to HvHOX1, HvHOX2 is more highly conserved across different barley genotypes and Hordeum species, hinting at a possibly vital but still unclarified biological role. Using bimolecular fluorescence complementation, DNA-binding, and transactivation assays, we validate that HvHOX1 and HvHOX2 are bona fide transcriptional activators that may potentially heterodimerize. Accordingly, both genes exhibit similar spatiotemporal expression patterns during spike development and growth, albeit their mRNA levels differ quantitatively. We show that HvHOX1 delays the lateral spikelet meristem differentiation and affects fertility by aborting the reproductive organs. Interestingly, the ancestral relationship of these genes inferred from their co-expressed gene networks suggested that HvHOX1 and HvHOX2 might play a similar role during barley spikelet development. However, CRISPR-derived mutants of HvHOX1 and HvHOX2 demonstrated the suppressive role of HvHOX1 on lateral spikelets, while the loss of HvHOX2 does not influence spikelet development. Collectively, our study shows that through the suppression of reproductive organs, lateral spikelet fertility is regulated by HvHOX1, whereas HvHOX2 is dispensable for spikelet development in barley.
1000 Sacherschließung
lokal CRISPR
lokal gene duplication
lokal nucleotide diversity
lokal HD-ZIP class I transcription factors
lokal spikelet fertility
lokal inflorescence architecture
lokal Hordeum vulgare
lokal anther and pistil development
lokal barley
1000 Fächerklassifikation (DDC)
1000 Liste der Beteiligten
  1. https://orcid.org/0000-0002-7814-5475|https://orcid.org/0000-0003-0450-0814|https://orcid.org/0000-0002-5539-3097|https://orcid.org/0000-0003-0665-5094|https://orcid.org/0000-0003-3104-0825|https://orcid.org/0000-0001-8907-6922|https://orcid.org/0000-0001-5891-6503|https://orcid.org/0000-0001-8566-9501|https://orcid.org/0000-0003-0946-0508|https://orcid.org/0000-0002-5341-187X|https://orcid.org/0000-0001-7181-9855|https://orcid.org/0000-0003-1622-5346|https://orcid.org/0000-0003-2951-0541|https://orcid.org/0000-0001-8269-7494|https://orcid.org/0000-0001-7080-7983|https://orcid.org/0000-0001-8195-249X|https://orcid.org/0000-0002-5267-0677|https://orcid.org/0000-0002-3998-038X
1000 Label
1000 Förderer
  1. Deutsche Forschungsgemeinschaft |
  2. Deutsche Forschungsgemeinschaft |
  3. Bundesministerium für Bildung und Forschung |
  4. European Research Council |
1000 Fördernummer
  1. SCHN 768/4–1
  2. SCHN 768/15-1
  3. 0315071
  4. 681686;ERC-2015-CoG
1000 Förderprogramm
  1. -
  2. HEISENBERG Program
  3. GABIFUTURE
  4. LUSH SPIK0045
1000 Dateien
1000 Förderung
  1. 1000 joinedFunding-child
    1000 Förderer Deutsche Forschungsgemeinschaft |
    1000 Förderprogramm -
    1000 Fördernummer SCHN 768/4–1
  2. 1000 joinedFunding-child
    1000 Förderer Deutsche Forschungsgemeinschaft |
    1000 Förderprogramm HEISENBERG Program
    1000 Fördernummer SCHN 768/15-1
  3. 1000 joinedFunding-child
    1000 Förderer Bundesministerium für Bildung und Forschung |
    1000 Förderprogramm GABIFUTURE
    1000 Fördernummer 0315071
  4. 1000 joinedFunding-child
    1000 Förderer European Research Council |
    1000 Förderprogramm LUSH SPIK0045
    1000 Fördernummer 681686;ERC-2015-CoG
1000 Objektart article
1000 Beschrieben durch
1000 @id frl:6489135.rdf
1000 Erstellt am 2024-11-22T14:26:37.198+0100
1000 Erstellt von 325
1000 beschreibt frl:6489135
1000 Bearbeitet von 317
1000 Zuletzt bearbeitet 2025-02-24T14:29:34.132+0100
1000 Objekt bearb. Mon Feb 24 14:29:23 CET 2025
1000 Vgl. frl:6489135
1000 Oai Id
  1. oai:frl.publisso.de:frl:6489135 |
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