Barley Ror1 encodes a class XI myosin required for mlo-based broad-spectrum resistance to the fungal powdery mildew pathogen

  1. Acevedo-Garcia, Johanna ORCID logo
  2. Walden, Kim
  3. Leissing, Franz ORCID logo
  4. Baumgarten, Kira
  5. Drwiega, Katarzyna
  6. Kwaaitaal, Mark
  7. Reinstädler, Anja
  8. Freh, Matthias
  9. Dong, Xue
  10. James, Geo Velikkakam
  11. Baus, Lisa C
  12. Mascher, Martin ORCID logo
  13. Stein, Nils ORCID logo
  14. Schneeberger, Korbinian ORCID logo
  15. Brocke-Ahmadinejad, Nahal
  16. Kollmar, Martin ORCID logo
  17. Schulze-Lefert, Paul ORCID logo
  18. Panstruga, Ralph ORCID logo

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Acevedo-Garcia-Plant J-2022.pdf 1,48MB
WeightNameValue
1000 Titel
  • Barley Ror1 encodes a class XI myosin required for mlo-based broad-spectrum resistance to the fungal powdery mildew pathogen
1000 Autor/in
  1. Acevedo-Garcia, Johanna |
  2. Walden, Kim |
  3. Leissing, Franz |
  4. Baumgarten, Kira |
  5. Drwiega, Katarzyna |
  6. Kwaaitaal, Mark |
  7. Reinstädler, Anja |
  8. Freh, Matthias |
  9. Dong, Xue |
  10. James, Geo Velikkakam |
  11. Baus, Lisa C |
  12. Mascher, Martin |
  13. Stein, Nils |
  14. Schneeberger, Korbinian |
  15. Brocke-Ahmadinejad, Nahal |
  16. Kollmar, Martin |
  17. Schulze-Lefert, Paul |
  18. Panstruga, Ralph |
1000 Erscheinungsjahr 2022
1000 LeibnizOpen
1000 Publikationstyp
  1. Artikel |
1000 Online veröffentlicht
  • 2022-08-02
1000 Erschienen in
1000 Quellenangabe
  • 112(1):84-103
1000 FRL-Sammlung
1000 Copyrightjahr
  • 2022
1000 Lizenz
1000 Verlagsversion
  • https://dx.doi.org/10.1111/tpj.15930 |
1000 Publikationsstatus
1000 Begutachtungsstatus
1000 Sprache der Publikation
1000 Abstract/Summary
  • Loss-of-function alleles of plant MLO genes confer broad-spectrum resistance to powdery mildews in many eudicot and monocot plant species. Although barley mlo mutants have been used in agriculture for more than 40 years, understanding of the molecular principles underlying this type of disease resistance remains fragmentary. Forward genetic screens in barley have revealed mutations in two Required for mlo resistance (Ror) genes that partially impair immunity conferred by mlo mutants. While Ror2 encodes a soluble N-ethylmaleimide-sensitive factor attached protein receptor (SNARE), the identity of Ror1, located at the pericentromeric region of barley chromosome 1H, remained elusive. We report the identification of Ror1 based on combined barley genomic sequence information and transcriptomic data from ror1 mutant plants. Ror1 encodes the barley class XI myosin Myo11A (HORVU.MOREX.r3.1HG0046420). Single amino acid substitutions of this myosin, deduced from non-functional ror1 mutant alleles, map to the nucleotide-binding region and the interface between the relay-helix and the converter domain of the motor protein. Ror1 myosin accumulates transiently in the course of powdery mildew infection. Functional fluorophore-labeled Ror1 variants associate with mobile intracellular compartments that partially colocalize with peroxisomes. Single-cell expression of the Ror1 tail region causes a dominant-negative effect that phenocopies ror1 loss-of-function mutants. We define a myosin motor for the establishment of mlo-mediated resistance, suggesting that motor protein-driven intracellular transport processes are critical for extracellular immunity, possibly through the targeted transfer of antifungal and/or cell wall cargoes to pathogen contact sites.
1000 Sacherschließung
lokal suppressor mutant
lokal Blumeria graminis f.sp. hordei
lokal powdery mildew
lokal mlo resistance
lokal myosin XI
lokal Hordeum vulgare
lokal barley
1000 Fächerklassifikation (DDC)
1000 Liste der Beteiligten
  1. https://orcid.org/0000-0001-5797-8681|https://frl.publisso.de/adhoc/uri/V2FsZGVuLCBLaW0=|https://orcid.org/0000-0002-9746-7162|https://frl.publisso.de/adhoc/uri/QmF1bWdhcnRlbiwgS2lyYQ==|https://frl.publisso.de/adhoc/uri/RHJ3aWVnYSwgS2F0YXJ6eW5h|https://frl.publisso.de/adhoc/uri/S3dhYWl0YWFsLCBNYXJr|https://frl.publisso.de/adhoc/uri/UmVpbnN0w6RkbGVyLCBBbmph|https://frl.publisso.de/adhoc/uri/RnJlaCwgTWF0dGhpYXM=|https://frl.publisso.de/adhoc/uri/RG9uZywgWHVl|https://frl.publisso.de/adhoc/uri/SmFtZXMsIEdlbyBWZWxpa2tha2Ft|https://frl.publisso.de/adhoc/uri/QmF1cywgTGlzYSBD|https://orcid.org/0000-0001-6373-6013|https://orcid.org/0000-0003-3011-8731|https://orcid.org/0000-0002-5512-0443|https://frl.publisso.de/adhoc/uri/QnJvY2tlLUFobWFkaW5lamFkLCBOYWhhbA==|https://orcid.org/0000-0002-9768-1855|https://orcid.org/0000-0002-8978-1717|https://orcid.org/0000-0002-3756-8957
1000 Label
1000 Förderer
  1. International Max Planck Research School |
  2. Novo Nordisk Fonden |
1000 Fördernummer
  1. -
  2. NF19OC0056457
1000 Förderprogramm
  1. -
  2. -
1000 Dateien
1000 Förderung
  1. 1000 joinedFunding-child
    1000 Förderer International Max Planck Research School |
    1000 Förderprogramm -
    1000 Fördernummer -
  2. 1000 joinedFunding-child
    1000 Förderer Novo Nordisk Fonden |
    1000 Förderprogramm -
    1000 Fördernummer NF19OC0056457
1000 Objektart article
1000 Beschrieben durch
1000 @id frl:6489206.rdf
1000 Erstellt am 2024-11-27T17:29:01.292+0100
1000 Erstellt von 325
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1000 Bearbeitet von 317
1000 Zuletzt bearbeitet 2025-02-26T10:23:07.293+0100
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