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1000
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Abstract/Summary
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<jats:title>Abstract</jats:title><jats:sec>
<jats:title>Background</jats:title>
<jats:p>Key functions of Ca<jats:sup>2+</jats:sup> signaling in rodent microglia include monitoring the brain state as well as the surrounding neuronal activity and sensing the danger or damage in their vicinity. Microglial Ca<jats:sup>2+</jats:sup> dyshomeostasis is a disease hallmark in many mouse models of neurological disorders but the Ca<jats:sup>2+</jats:sup> signal properties of human microglia remain unknown.</jats:p>
</jats:sec><jats:sec>
<jats:title>Methods</jats:title>
<jats:p>We developed a novel genetically-encoded ratiometric Ca<jats:sup>2+</jats:sup> indicator, targeting microglial cells in the freshly resected human tissue, organotypically cultured tissue slices and analyzed in situ ongoing Ca<jats:sup>2+</jats:sup> signaling of decades-old microglia dwelling in their native microenvironment.</jats:p>
</jats:sec><jats:sec>
<jats:title>Results</jats:title>
<jats:p>The data revealed marked compartmentalization of Ca<jats:sup>2+</jats:sup> signals, with signal properties differing across the compartments and resident morphotypes. The basal Ca<jats:sup>2+</jats:sup> levels were low in ramified and high in ameboid microglia. The fraction of cells with ongoing Ca<jats:sup>2+</jats:sup> signaling, the fraction and the amplitude of process Ca<jats:sup>2+</jats:sup> signals and the duration of somatic Ca<jats:sup>2+</jats:sup> signals decreased when moving from ramified via hypertrophic to ameboid microglia. In contrast, the size of active compartments, the fraction and amplitude of somatic Ca<jats:sup>2+</jats:sup> signals and the duration of process Ca<jats:sup>2+</jats:sup> signals increased along this pathway.</jats:p>
</jats:sec>
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1000
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Hinweis
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DeepGreen-ID: b3c4c83d8f6f4ed4b657a4de632a76c7 ; metadata provieded by: DeepGreen (https://www.oa-deepgreen.de/api/v1/), LIVIVO search scope life sciences (http://z3950.zbmed.de:6210/livivo), Crossref Unified Resource API (https://api.crossref.org/swagger-ui/index.html), to.science.api (https://frl.publisso.de/), ZDB JSON-API (beta) (https://zeitschriftendatenbank.de/api/), lobid - Dateninfrastruktur für Bibliotheken (https://lobid.org/resources/search)
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