17α-Ethynylestradiol alters testicular epigenetic profiles and histone-to-protamine exchange in mice

  1. Ded, L.
  2. Zatecka-Lanska, E.
  3. Vaculikova, E.
  4. Frolikova, M.
  5. Sanovec, O.
  6. Palenikova, V.
  7. Simonik, O.
  8. Dorosh, A.
  9. Margaryan, H.
  10. Elzeinova, F.
  11. Kubatova, A.
  12. Peknicova, J.
  13. Paradowska-Dogan, A.
  14. Steger, K.
  15. Komrskova, K.

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WeightNameValue
1000 Titel
  • 17α-Ethynylestradiol alters testicular epigenetic profiles and histone-to-protamine exchange in mice
1000 Autor/in
  1. Ded, L. |
  2. Zatecka-Lanska, E. |
  3. Vaculikova, E. |
  4. Frolikova, M. |
  5. Sanovec, O. |
  6. Palenikova, V. |
  7. Simonik, O. |
  8. Dorosh, A. |
  9. Margaryan, H. |
  10. Elzeinova, F. |
  11. Kubatova, A. |
  12. Peknicova, J. |
  13. Paradowska-Dogan, A. |
  14. Steger, K. |
  15. Komrskova, K. |
1000 Verlag BioMed Central
1000 Erscheinungsjahr 2024
1000 Publikationstyp
  1. Artikel |
1000 Online veröffentlicht
  • 2024-11-04
1000 Erschienen in
1000 Quellenangabe
  • 22(1):135
1000 Copyrightjahr
  • 2024
1000 Lizenz
1000 Verlagsversion
  • https://doi.org/10.1186/s12958-024-01307-6 |
  • https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11533287/ |
1000 Publikationsstatus
1000 Begutachtungsstatus
1000 Sprache der Publikation
1000 Abstract/Summary
  • Spermatogenesis starts with the onset of puberty within the seminiferous epithelium of the testes. It is a complex process under intricate control of the endocrine system. Physiological regulations by steroid hormones in general and by estrogens in particular are due to their chemical nature prone to be disrupted by exogenous factors acting as endocrine disruptors (EDs). 17α-Ethynylestradiol (EE2) is an environmental pollutant with a confirmed ED activity and a well-known effect on spermatogenesis and chromatin remodeling in haploid germ cells. The aim of our study was to assess possible effects of two doses (2.5ng/ml; 2.5 μg/ml) of EE2 on both histone-to-protamine exchange and epigenetic profiles during spermatogenesis performing a multi/transgenerational study in mice. Our results demonstrated an impaired histone-to-protamine exchange with a significantly higher histone retention in sperm nuclei of exposed animals, when this process was accompanied by the changes of histone post-translational modifications (PTMs) abundancies with a prominent effect on H3K9Ac and partial changes in protamine 1 promoter methylation status. Furthermore, individual changes in molecular phenotypes were partially transmitted to subsequent generations, when no direct trans-generational effect was observed. Finally, the uncovered specific localization of the histone retention in sperm nuclei and their specific PTMs profile after EE2 exposure may indicate an estrogenic effect on sperm motility and early embryonic development via epigenetic mechanisms.
1000 Sacherschließung
lokal Protamines/metabolism [MeSH]
lokal Endocrine Disruptors/pharmacology [MeSH]
lokal Protamines/genetics [MeSH]
lokal Testis
lokal EE2
lokal Male [MeSH]
lokal Testis/metabolism [MeSH]
lokal Ethinyl Estradiol/pharmacology [MeSH]
lokal Endocrine Disruptors/toxicity [MeSH]
lokal DNA methylation
lokal Sperm
lokal Female [MeSH]
lokal Histones/metabolism [MeSH]
lokal Spermatogenesis/genetics [MeSH]
lokal Protein Processing, Post-Translational/drug effects [MeSH]
lokal Animals [MeSH]
lokal Endocrine disruptors
lokal Spermatozoa/drug effects [MeSH]
lokal Transgenerational study
lokal Histone-to-protamine exchange
lokal Mice [MeSH]
lokal Spermatozoa/metabolism [MeSH]
lokal Post-translational modifications
lokal Testis/drug effects [MeSH]
lokal Spermatogenesis/drug effects [MeSH]
lokal Research
lokal 17α-Ethynylestradiol
lokal Epigenesis, Genetic/drug effects [MeSH]
1000 Fächerklassifikation (DDC)
1000 Liste der Beteiligten
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  1. Grantová Agentura České Republiky |
  2. Deutsche Forschungsgemeinschaft |
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    1000 Förderer Deutsche Forschungsgemeinschaft |
    1000 Förderprogramm -
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1000 Erstellt am 2025-02-06T21:10:50.901+0100
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