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1000 Titel
  • FBLN2 is associated with basal cell markers Krt14 and ITGB1 in mouse mammary epithelial cells and has a preferential expression in molecular subtypes of human breast cancer
1000 Autor/in
  1. WalyEldeen, Amr Ahmed |
  2. Sabet, Salwa |
  3. Anis, Shady E. |
  4. Stein, Torsten |
  5. Ibrahim, Ayman M. |
1000 Verlag Springer US
1000 Erscheinungsjahr 2024
1000 Publikationstyp
  1. Artikel |
1000 Online veröffentlicht
  • 2024-08-07
1000 Erschienen in
1000 Quellenangabe
  • 208(3):673-686
1000 Copyrightjahr
  • 2024
1000 Lizenz
1000 Verlagsversion
  • https://doi.org/10.1007/s10549-024-07447-y |
  • https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11522036/ |
1000 Publikationsstatus
1000 Begutachtungsstatus
1000 Sprache der Publikation
1000 Abstract/Summary
  • <jats:title>Abstract</jats:title><jats:sec> <jats:title>Background</jats:title> <jats:p>Fibulin-2 (FBLN2) is a secreted extracellular matrix (ECM) glycoprotein and has been identified in the mouse mammary gland, in cap cells of terminal end buds (TEBs) during puberty, and around myoepithelial cells during early pregnancy. It is required for basement membrane (BM) integrity in mammary epithelium, and its loss has been associated with human breast cancer invasion. Herein, we attempted to confirm the relevance of FBLN2 to myoepithelial phenotype in mammary epithelium and to assess its expression in molecular subtypes of human breast cancer.</jats:p> </jats:sec><jats:sec> <jats:title>Methods</jats:title> <jats:p>The relationship between FBLN2 expression and epithelial markers was investigated in pubertal mouse mammary glands and the EpH4 mouse mammary epithelial cell line using immunohistochemistry, immunocytochemistry, and immunoblotting. Human breast cancer mRNA data from the METABRIC and TCGA datasets from Bioportal were analyzed to assess the association of <jats:italic>Fbln2</jats:italic> expression with epithelial markers, and with molecular subtypes. Survival curves were generated using data from the METABRIC dataset and the KM databases.</jats:p> </jats:sec><jats:sec> <jats:title>Results</jats:title> <jats:p>FBLN2 knockdown in mouse mammary epithelial cells was associated with a reduction in KRT14 and an increase in KRT18. Further, TGFβ3 treatment resulted in the upregulation of FBLN2 in vitro. Meta-analyses of human breast cancer datasets from Bioportal showed a higher expression of <jats:italic>Fbln2</jats:italic> mRNA in claudin-low, LumA, and normal-like breast cancers compared to LumB, Her2 +, and Basal-like subgroups. <jats:italic>Fbln2</jats:italic> mRNA levels were positively associated with mesenchymal markers, myoepithelial markers, and markers of epithelial–mesenchymal transition. Higher expression of <jats:italic>Fbln2</jats:italic> mRNA was associated with better prognosis in less advanced breast cancer and this pattern was reversed in more advanced lesions.</jats:p> </jats:sec><jats:sec> <jats:title>Conclusion</jats:title> <jats:p>With further validation, these observations may offer a molecular prognostic tool for human breast cancer for more personalized therapeutic approaches.</jats:p> </jats:sec>
1000 Sacherschließung
lokal Gene Expression Regulation, Neoplastic [MeSH]
lokal Molecular subtypes
lokal Cell Line, Tumor [MeSH]
lokal Keratin-14/metabolism [MeSH]
lokal Extracellular Matrix Proteins/genetics [MeSH]
lokal Breast cancer
lokal Mammary Glands, Animal/pathology [MeSH]
lokal ECM
lokal Epithelial Cells/metabolism [MeSH]
lokal Keratin-14/genetics [MeSH]
lokal Fibulin-2
lokal Basal
lokal Mammary Glands, Animal/metabolism [MeSH]
lokal Calcium-Binding Proteins/metabolism [MeSH]
lokal Extracellular Matrix Proteins/metabolism [MeSH]
lokal Epithelial Cells/pathology [MeSH]
lokal Breast Neoplasms/pathology [MeSH]
lokal Female [MeSH]
lokal Humans [MeSH]
lokal Breast Neoplasms/genetics [MeSH]
lokal Breast Neoplasms/metabolism [MeSH]
lokal Animals [MeSH]
lokal Calcium-Binding Proteins/genetics [MeSH]
lokal Mice [MeSH]
lokal Biomarkers, Tumor/metabolism [MeSH]
lokal Research
lokal Biomarkers, Tumor/genetics [MeSH]
1000 Fächerklassifikation (DDC)
1000 Liste der Beteiligten
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  1. Cairo University |
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    1000 Förderer Cairo University |
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1000 Erstellt am 2025-07-03T23:40:57.759+0200
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