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Abstract/Summary
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<jats:title>Abstract</jats:title><jats:sec>
<jats:title>Background</jats:title>
<jats:p>Bacterial pathogens such as <jats:italic>Staphylococcus aureus</jats:italic> colonize body surfaces of part of the human population, which represents a critical risk factor for skin disorders and invasive infections. However, such pathogens do not belong to the human core microbiomes. Beneficial commensal bacteria can often prevent the invasion and persistence of such pathogens by using molecular strategies that are only superficially understood. We recently reported that the commensal bacterium <jats:italic>Staphylococcus lugdunensis</jats:italic> produces the novel antibiotic lugdunin, which eradicates <jats:italic>S. aureus</jats:italic> from the nasal microbiomes of hospitalized patients. However, it has remained unclear if <jats:italic>S. lugdunensis</jats:italic> may affect <jats:italic>S. aureus</jats:italic> carriage in the general population and which external factors might promote <jats:italic>S. lugdunensis</jats:italic> carriage to enhance its <jats:italic>S. aureus</jats:italic>-eliminating capacity.</jats:p>
</jats:sec><jats:sec>
<jats:title>Results</jats:title>
<jats:p>We could cultivate <jats:italic>S. lugdunensis</jats:italic> from the noses of 6.3% of healthy human volunteers. In addition, <jats:italic>S. lugdunensis</jats:italic> DNA could be identified in metagenomes of many culture-negative nasal samples indicating that cultivation success depends on a specific bacterial threshold density. Healthy <jats:italic>S. lugdunensis</jats:italic> carriers had a 5.2-fold lower propensity to be colonized by <jats:italic>S. aureus</jats:italic> indicating that lugdunin can eliminate <jats:italic>S. aureus</jats:italic> also in healthy humans. <jats:italic>S. lugdunensis</jats:italic>-positive microbiomes were dominated by either <jats:italic>Staphylococcus epidermidis</jats:italic>, <jats:italic>Corynebacterium</jats:italic> species, or <jats:italic>Dolosigranulum pigrum</jats:italic>. These and further bacterial commensals, whose abundance was positively associated with <jats:italic>S. lugdunensis</jats:italic>, promoted <jats:italic>S. lugdunensis</jats:italic> growth in co-culture. Such mutualistic interactions depended on the production of iron-scavenging siderophores by supportive commensals and on the capacity of <jats:italic>S. lugdunensis</jats:italic> to import siderophores.</jats:p>
</jats:sec><jats:sec>
<jats:title>Conclusions</jats:title>
<jats:p>These findings underscore the importance of microbiome homeostasis for eliminating pathogen colonization. Elucidating mechanisms that drive microbiome interactions will become crucial for microbiome-precision editing approaches.</jats:p>
</jats:sec>
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Hinweis
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DeepGreen-ID: f847cd44811c4e6d80bb25240f9e5576 ; metadata provieded by: DeepGreen (https://www.oa-deepgreen.de/api/v1/), LIVIVO search scope life sciences (http://z3950.zbmed.de:6210/livivo), Crossref Unified Resource API (https://api.crossref.org/swagger-ui/index.html), to.science.api (https://frl.publisso.de/), ZDB JSON-API (beta) (https://zeitschriftendatenbank.de/api/), lobid - Dateninfrastruktur für Bibliotheken (https://lobid.org/resources/search)
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