Detection of polyreactive immunoglobulin G facilitates diagnosis in children with autoimmune hepatitis

  1. Engel, Bastian ORCID logo
  2. Diestelhorst, Jana
  3. Hupa-Breier, Katharina Luise
  4. Kirchner, Theresa
  5. Henjes, Nicole
  6. Loges, Stephanie
  7. Yuksel, Muhammed
  8. Janczyk, Wojciech
  9. Lalanne, Claudine
  10. Zachou, Kalliopi
  11. Oo, Ye H.
  12. Gournay, Jérôme
  13. Pape, Simon
  14. Drenth, Joost P. H.
  15. Renand, Amédée
  16. Dalekos, George N.
  17. Muratori, Luigi
  18. Socha, Piotr
  19. Ma, Yun
  20. Arikan, Cigdem
  21. Baumann, Ulrich
  22. Manns, Michael P.
  23. Wedemeyer, Heiner
  24. Junge, Norman
  25. Jaeckel, Elmar
  26. Taubert, Richard

Download
12072_2024_Article_10695.pdf 2,11MB
WeightNameValue
1000 Titel
  • Detection of polyreactive immunoglobulin G facilitates diagnosis in children with autoimmune hepatitis
1000 Autor/in
  1. Engel, Bastian |
  2. Diestelhorst, Jana |
  3. Hupa-Breier, Katharina Luise |
  4. Kirchner, Theresa |
  5. Henjes, Nicole |
  6. Loges, Stephanie |
  7. Yuksel, Muhammed |
  8. Janczyk, Wojciech |
  9. Lalanne, Claudine |
  10. Zachou, Kalliopi |
  11. Oo, Ye H. |
  12. Gournay, Jérôme |
  13. Pape, Simon |
  14. Drenth, Joost P. H. |
  15. Renand, Amédée |
  16. Dalekos, George N. |
  17. Muratori, Luigi |
  18. Socha, Piotr |
  19. Ma, Yun |
  20. Arikan, Cigdem |
  21. Baumann, Ulrich |
  22. Manns, Michael P. |
  23. Wedemeyer, Heiner |
  24. Junge, Norman |
  25. Jaeckel, Elmar |
  26. Taubert, Richard |
1000 Verlag Springer India
1000 Erscheinungsjahr 2024
1000 Publikationstyp
  1. Artikel |
1000 Online veröffentlicht
  • 2024-07-08
1000 Erschienen in
1000 Quellenangabe
  • 18(4):1214-1226
1000 Copyrightjahr
  • 2024
1000 Lizenz
1000 Verlagsversion
  • https://doi.org/10.1007/s12072-024-10695-1 |
  • https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11297808/ |
1000 Publikationsstatus
1000 Begutachtungsstatus
1000 Sprache der Publikation
1000 Abstract/Summary
  • <jats:title>Abstract</jats:title><jats:sec> <jats:title>Objective</jats:title> <jats:p>The detection of autoantibodies is essential to diagnose autoimmune hepatitis (AIH). Particularly in children, specificity of autoantibodies decreases due to lower titers being diagnostic and being present not only in AIH but also in other liver diseases. Recently, quantification of polyreactive IgG (pIgG) for detection of adult AIH showed the highest overall accuracy compared to antinuclear antibodies (ANA), anti-smooth muscle antibodies (anti-SMA), anti-liver kidney microsomal antibodies (anti-LKM) and anti-soluble liver antigen/liver pancreas antibodies (anti-SLA/LP). We aimed to evaluate the diagnostic value of pIgG for pediatric AIH.</jats:p> </jats:sec><jats:sec> <jats:title>Design</jats:title> <jats:p>pIgG, quantified using HIP1R/BSA coated ELISA, and immunofluorescence on rodent tissue sections were performed centrally. The diagnostic fidelity to diagnose AIH was compared to conventional autoantibodies of AIH in training and validation cohorts from a retrospective, European multi-center cohort from nine centers from eight European countries composed of existing biorepositories from expert centers (<jats:italic>n</jats:italic> = 285).</jats:p> </jats:sec><jats:sec> <jats:title>Results</jats:title> <jats:p>IgG from pediatric AIH patients exhibited increased polyreactivity to multiple protein and non-protein substrates compared to non-AIH liver diseases and healthy children. pIgG had an AUC of 0.900 to distinguish AIH from non-AIH liver diseases. pIgG had a 31–73% higher specificity than ANA and anti-SMA and comparable sensitivity that was 6–20 times higher than of anti-SLA/LP, anti-LC1 and anti-LKM. pIgG had a 21–34% higher accuracy than conventional autoantibodies, was positive in 43–75% of children with AIH and normal IgG and independent from treatment response.</jats:p> </jats:sec><jats:sec> <jats:title>Conclusion</jats:title> <jats:p>Detecting pIgG improves the diagnostic evaluation of pediatric AIH compared to conventional autoantibodies, primarily owing to higher accuracy and specificity.</jats:p> </jats:sec><jats:sec> <jats:title>Graphical Abstract</jats:title> </jats:sec>
1000 Sacherschließung
lokal Anti-smooth muscle antibody
lokal Adolescent [MeSH]
lokal Female [MeSH]
lokal Antinuclear antibody
lokal Autoantibodies/blood [MeSH]
lokal Humans [MeSH]
lokal Retrospective Studies [MeSH]
lokal Anti-soluble liver antigen/liver pancreas
lokal Animals [MeSH]
lokal Autoantibodies/immunology [MeSH]
lokal Hepatitis, Autoimmune/immunology [MeSH]
lokal Immunoglobulin G/immunology [MeSH]
lokal Original Article
lokal Huntingtin-interacting protein 1-related protein (HIP1R)
lokal Sensitivity and Specificity [MeSH]
lokal Anti-liver kidney microsomal antibody
lokal Male [MeSH]
lokal Hepatitis, Autoimmune/blood [MeSH]
lokal Enzyme-Linked Immunosorbent Assay/methods [MeSH]
lokal Child [MeSH]
lokal Hepatitis, Autoimmune/diagnosis [MeSH]
lokal Immunoglobulin G/blood [MeSH]
lokal Child, Preschool [MeSH]
1000 Fächerklassifikation (DDC)
1000 Liste der Beteiligten
  1. https://orcid.org/0000-0002-0972-3454|https://frl.publisso.de/adhoc/uri/RGllc3RlbGhvcnN0LCBKYW5h|https://frl.publisso.de/adhoc/uri/SHVwYS1CcmVpZXIsIEthdGhhcmluYSBMdWlzZQ==|https://frl.publisso.de/adhoc/uri/S2lyY2huZXIsIFRoZXJlc2E=|https://frl.publisso.de/adhoc/uri/SGVuamVzLCBOaWNvbGU=|https://frl.publisso.de/adhoc/uri/TG9nZXMsIFN0ZXBoYW5pZQ==|https://frl.publisso.de/adhoc/uri/WXVrc2VsLCBNdWhhbW1lZA==|https://frl.publisso.de/adhoc/uri/SmFuY3p5aywgV29qY2llY2g=|https://frl.publisso.de/adhoc/uri/TGFsYW5uZSwgQ2xhdWRpbmU=|https://frl.publisso.de/adhoc/uri/WmFjaG91LCBLYWxsaW9waQ==|https://frl.publisso.de/adhoc/uri/T28sIFllIEgu|https://frl.publisso.de/adhoc/uri/R291cm5heSwgSsOpcsO0bWU=|https://frl.publisso.de/adhoc/uri/UGFwZSwgU2ltb24=|https://frl.publisso.de/adhoc/uri/RHJlbnRoLCBKb29zdCBQLiBILg==|https://frl.publisso.de/adhoc/uri/UmVuYW5kLCBBbcOpZMOpZQ==|https://frl.publisso.de/adhoc/uri/RGFsZWtvcywgR2VvcmdlIE4u|https://frl.publisso.de/adhoc/uri/TXVyYXRvcmksIEx1aWdp|https://frl.publisso.de/adhoc/uri/U29jaGEsIFBpb3Ry|https://frl.publisso.de/adhoc/uri/TWEsIFl1bg==|https://frl.publisso.de/adhoc/uri/QXJpa2FuLCBDaWdkZW0=|https://frl.publisso.de/adhoc/uri/QmF1bWFubiwgVWxyaWNo|https://frl.publisso.de/adhoc/uri/TWFubnMsIE1pY2hhZWwgUC4=|https://frl.publisso.de/adhoc/uri/V2VkZW1leWVyLCBIZWluZXI=|https://frl.publisso.de/adhoc/uri/SnVuZ2UsIE5vcm1hbg==|https://frl.publisso.de/adhoc/uri/SmFlY2tlbCwgRWxtYXI=|https://frl.publisso.de/adhoc/uri/VGF1YmVydCwgUmljaGFyZA==
1000 Hinweis
  • DeepGreen-ID: c2ab05be65a441cbaef4464a29af557c ; metadata provieded by: DeepGreen (https://www.oa-deepgreen.de/api/v1/), LIVIVO search scope life sciences (http://z3950.zbmed.de:6210/livivo), Crossref Unified Resource API (https://api.crossref.org/swagger-ui/index.html), to.science.api (https://frl.publisso.de/), ZDB JSON-API (beta) (https://zeitschriftendatenbank.de/api/), lobid - Dateninfrastruktur für Bibliotheken (https://lobid.org/resources/search)
1000 Label
1000 Förderer
  1. Deutsche Forschungsgemeinschaft |
  2. Horizon 2020 Framework Programme |
  3. Else Kröner-Fresenius-Stiftung |
  4. Medizinischen Hochschule Hannover |
  5. Sir Jules Thorn Charitable Trust |
  6. Medizinische Hochschule Hannover (MHH) |
1000 Fördernummer
  1. -
  2. -
  3. -
  4. -
  5. -
  6. -
1000 Förderprogramm
  1. -
  2. -
  3. -
  4. -
  5. -
  6. -
1000 Dateien
1000 Förderung
  1. 1000 joinedFunding-child
    1000 Förderer Deutsche Forschungsgemeinschaft |
    1000 Förderprogramm -
    1000 Fördernummer -
  2. 1000 joinedFunding-child
    1000 Förderer Horizon 2020 Framework Programme |
    1000 Förderprogramm -
    1000 Fördernummer -
  3. 1000 joinedFunding-child
    1000 Förderer Else Kröner-Fresenius-Stiftung |
    1000 Förderprogramm -
    1000 Fördernummer -
  4. 1000 joinedFunding-child
    1000 Förderer Medizinischen Hochschule Hannover |
    1000 Förderprogramm -
    1000 Fördernummer -
  5. 1000 joinedFunding-child
    1000 Förderer Sir Jules Thorn Charitable Trust |
    1000 Förderprogramm -
    1000 Fördernummer -
  6. 1000 joinedFunding-child
    1000 Förderer Medizinische Hochschule Hannover (MHH) |
    1000 Förderprogramm -
    1000 Fördernummer -
1000 Objektart article
1000 Beschrieben durch
1000 @id frl:6519095.rdf
1000 Erstellt am 2025-07-05T15:37:09.737+0200
1000 Erstellt von 322
1000 beschreibt frl:6519095
1000 Zuletzt bearbeitet 2025-08-11T11:55:32.099+0200
1000 Objekt bearb. Mon Aug 11 11:55:32 CEST 2025
1000 Vgl. frl:6519095
1000 Oai Id
  1. oai:frl.publisso.de:frl:6519095 |
1000 Sichtbarkeit Metadaten public
1000 Sichtbarkeit Daten public
1000 Gegenstand von

View source