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1000
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Abstract/Summary
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<jats:title>Abstract</jats:title><jats:p>The recent emergence of chronic wasting disease (CWD) in Europe has become a new public health risk for monitoring of wild and farmed cervids. This disease, due to prions, has proliferated in North America in a contagious manner. In several mammalian species, polymorphisms in the prion protein gene (<jats:italic>PRNP</jats:italic>) play a crucial role in the susceptibility to prions and their spread. To obtain a reliable picture of the distribution of <jats:italic>PRNP</jats:italic> polymorphisms in the two most common cervid species in France, we sequenced the open reading frame (ORF) of this gene in 2114 animals, 1116 roe deer (<jats:italic>Capreolus capreolus</jats:italic>) and 998 red deer (<jats:italic>Cervus elaphus</jats:italic>). Selection criteria such as historical origin, spatial distribution and sex ratio have been integrated to establish this sample collection. Except for one heterozygous animal with a non-synonymous mutation at codon 37 (G37A), all the 1116 French roe deer were monomorphic. Red deer showed greater variation with two non-synonymous substitutions (T98A; Q226E), three synonymous substitutions (codons 21, 78 and 136) and a new 24pb deletion (Δ<jats:sub>69-77</jats:sub>). We found significant regional variations between French regions in the frequency of the identified substitutions. After cloning of the <jats:italic>PRNP</jats:italic> ORF from animals presenting multiple non-synonymous polymorphisms, we identified six haplotypes and obtained a total of twelve genotypes. As in other European countries, we highlighted the apparent homogeneity of <jats:italic>PRNP</jats:italic> in the French roe deer and the existence of a greater diversity in the red deer. These results were in line with European phylogeographic studies on these two species.</jats:p>
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1000
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Hinweis
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DeepGreen-ID: 8bd878b5fd784c069332255bcb5298b5 ; metadata provieded by: DeepGreen (https://www.oa-deepgreen.de/api/v1/), LIVIVO search scope life sciences (http://z3950.zbmed.de:6210/livivo), Crossref Unified Resource API (https://api.crossref.org/swagger-ui/index.html), to.science.api (https://frl.publisso.de/), ZDB JSON-API (beta) (https://zeitschriftendatenbank.de/api/), lobid - Dateninfrastruktur für Bibliotheken (https://lobid.org/resources/search)
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