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Abstract/Summary
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<jats:title>Abstract</jats:title><jats:sec>
<jats:title>Purpose</jats:title>
<jats:p><jats:italic>Cryptosporidium</jats:italic> is an intestinal zoonotic protozoan parasite that infects domesticated and wild animals. There are no reports on the prevalence and molecular characterisation of <jats:italic>Cryptosporidium</jats:italic> in the endemic Cyprus mouflon. The mouflon is strictly protected by national and international legislation. Its main distribution is Paphos State Forest and surrounding areas, where it may share the same water sources as free-ranging domestic goats. Therefore, the present study aimed to determine the prevalence of <jats:italic>Cryptosporidium</jats:italic> spp. and genotypes in mouflon and free-ranging goats within the mouflon range.</jats:p>
</jats:sec><jats:sec>
<jats:title>Methods</jats:title>
<jats:p>Faecal samples of 70 mouflons and 34 free-ranging goats were screened for <jats:italic>Cryptosporidium</jats:italic> by PCR amplification and sequencing.</jats:p>
</jats:sec><jats:sec>
<jats:title>Results</jats:title>
<jats:p>Only one sample (1/70) belonging to a mouflon was PCR positive for <jats:italic>Cryptosporidium</jats:italic>. Based on sequencing of the <jats:italic>18S</jats:italic> rRNA locus, this species was identified as <jats:italic>Cryptosporidium parvum</jats:italic> (<jats:italic>C. parvum</jats:italic>). No positive sample was detected in the free-ranging goats (0/34).</jats:p>
</jats:sec><jats:sec>
<jats:title>Conclusion</jats:title>
<jats:p>This is the first report on the molecular identification of this <jats:italic>Cryptosporidium</jats:italic> species in a Cyprus mouflon. The results indicate that the prevalence of <jats:italic>Cryptosporidium</jats:italic> in Cyprus mouflon is low.</jats:p>
</jats:sec>
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1000
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Hinweis
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DeepGreen-ID: 0b5b0d61852646b58a0f92e7b7534661 ; metadata provieded by: DeepGreen (https://www.oa-deepgreen.de/api/v1/), LIVIVO search scope life sciences (http://z3950.zbmed.de:6210/livivo), Crossref Unified Resource API (https://api.crossref.org/swagger-ui/index.html), to.science.api (https://frl.publisso.de/), ZDB JSON-API (beta) (https://zeitschriftendatenbank.de/api/), lobid - Dateninfrastruktur für Bibliotheken (https://lobid.org/resources/search)
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