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1000 Titel
  • A Multidisciplinary Approach toward High Throughput Label-Free Cytotoxicity Monitoring of Superparamagnetic Iron Oxide Nanoparticles
1000 Autor/in
  1. Tan, Sonia Abad |
  2. Zoidl, Georg |
  3. Ghafar-Zadeh, Ebrahim |
1000 Erscheinungsjahr 2019
1000 Publikationstyp
  1. Artikel |
1000 Online veröffentlicht
  • 2019-06-10
1000 Erschienen in
1000 Quellenangabe
  • 6(2):52
1000 Copyrightjahr
  • 2019
1000 Lizenz
1000 Verlagsversion
  • https://doi.org/10.3390/bioengineering6020052 |
1000 Publikationsstatus
1000 Begutachtungsstatus
1000 Sprache der Publikation
1000 Abstract/Summary
  • This paper focuses on cytotoxicity examination of superparamagnetic iron oxide nanoparticles (SPIONs) using different methods, including impedance spectroscopy. Recent advances of SPIONs for clinical and research applications have triggered the need to understand their effects in cells. Despite the great advances in adapting various biological and chemical methods to assess in-vitro toxicity of SPIONs, less attention has been paid on the development of a high throughput label-free screening platform to study the interaction between the cells and nanoparticles including SPIONs. In this paper, we have taken the first step toward this goal by proposing a label-free impedimetric method for monitoring living cells treated with SPIONs. We demonstrate the effect of SPIONs on the adhesion, growth, proliferation, and viability of neuroblastoma 2A (N2a) cells using impedance spectroscopy as a label-free method, along with other standard microscopic and cell viability testing methods as control methods. Our results have shown a decreased viability of the cells as the concentration of SPIONs increases with percentages of 59%, 47%, and 40% for 100 µg/mL (C4), 200 µg/mL (C5), 300 µg/mL (C6), respectively. Although all SPIONs concentrations have allowed the growth of cells within 72 h, C4, C5, and C6 showed slower growth compared to the control (C1). The growth and proliferation of N2a cells are faster in the absence or low concentration of SPIONS. The percent coefficient of variation (% CV) was used to compare cell concentrations obtained by TBDE assay and a Scepter cell counter. Results also showed that the lower the SPIONs concentration, the lower the impedance is expected to be in the sensing electrodes without the cells. Meanwhile, the variation of surface area (∆S) was affected by the concentration of SPIONs. It was observed that the double layer capacitance was almost constant because of the higher attachment of cells, the lower surface area coated by SPIONs. In conclusion, impedance changes of electrodes exposed to the mixture of cells and SPIONs offer a wide dynamic range (>1 MΩ using Electric Cell-substrate Impedance electrodes) suitable for cytotoxicity studies. Based on impedance based, viability testing and microscopic methods’ results, SPIONs concentrations higher than 100 ug/mL and 300 ug/mL cause minor and major effects, respectively. We propose that a high throughput impedance-based label-free platform provides great advantages for studying SPIONs in a cell-based context, opening a window of opportunity to design and test the next generation of SPIONs with reduced toxicity for biomedical or medical applications.
1000 Sacherschließung
lokal viability
lokal SPIONs
lokal neuroblastoma 2A
lokal high-throughput
lokal impedance-based assay
1000 Fächerklassifikation (DDC)
1000 Liste der Beteiligten
  1. https://frl.publisso.de/adhoc/uri/VGFuLCBTb25pYSBBYmFk|https://frl.publisso.de/adhoc/uri/Wm9pZGwsIEdlb3Jn|https://frl.publisso.de/adhoc/uri/R2hhZmFyLVphZGVoLCBFYnJhaGlt
1000 Label
1000 Förderer
  1. Natural Sciences and Engineering Research Council of Canada |
1000 Fördernummer
  1. DG 504129
1000 Förderprogramm
  1. -
1000 Dateien
1000 Förderung
  1. 1000 joinedFunding-child
    1000 Förderer Natural Sciences and Engineering Research Council of Canada |
    1000 Förderprogramm -
    1000 Fördernummer DG 504129
1000 Objektart article
1000 Beschrieben durch
1000 @id frl:6419900.rdf
1000 Erstellt am 2020-04-09T07:18:41.607+0200
1000 Erstellt von 21
1000 beschreibt frl:6419900
1000 Bearbeitet von 21
1000 Zuletzt bearbeitet 2020-04-09T07:20:36.635+0200
1000 Objekt bearb. Thu Apr 09 07:20:09 CEST 2020
1000 Vgl. frl:6419900
1000 Oai Id
  1. oai:frl.publisso.de:frl:6419900 |
1000 Sichtbarkeit Metadaten public
1000 Sichtbarkeit Daten public
1000 Gegenstand von

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