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1000 Titel
  • A reference genome of the European beech (Fagus sylvatica L.)
1000 Autor/in
  1. Mishra, Bagdevi |
  2. Gupta, Deepak K. |
  3. Pfenninger, Markus |
  4. Hickler, Thomas |
  5. Langer, Ewald |
  6. Nam, Bora |
  7. Paule, Juraj |
  8. SHARMA, RAHUL |
  9. Ulaszewski, Bartosz |
  10. Warmbier, Joanna |
  11. Burczyk, Jaroslaw |
  12. Thines, Marco |
1000 Erscheinungsjahr 2018
1000 LeibnizOpen
1000 Publikationstyp
  1. Artikel |
1000 Online veröffentlicht
  • 2018-05-28
1000 Erschienen in
1000 Quellenangabe
  • 7(6):giy063
1000 FRL-Sammlung
1000 Copyrightjahr
  • 2018
1000 Lizenz
1000 Verlagsversion
  • https://doi.org/10.1093/gigascience/giy063 |
  • https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6014182/ |
1000 Publikationsstatus
1000 Begutachtungsstatus
1000 Sprache der Publikation
1000 Abstract/Summary
  • BACKGROUND: The European beech is arguably the most important climax broad-leaved tree species in Central Europe, widely planted for its valuable wood. Here, we report the 542 Mb draft genome sequence of an up to 300-year-old individual (Bhaga) from an undisturbed stand in the Kellerwald-Edersee National Park in central Germany. FINDINGS: Using a hybrid assembly approach, Illumina reads with short- and long-insert libraries, coupled with long Pacific Biosciences reads, we obtained an assembled genome size of 542 Mb, in line with flow cytometric genome size estimation. The largest scaffold was of 1.15 Mb, the N50 length was 145 kb, and the L50 count was 983. The assembly contained 0.12% of Ns. A Benchmarking with Universal Single-Copy Orthologs (BUSCO) analysis retrieved 94% complete BUSCO genes, well in the range of other high-quality draft genomes of trees. A total of 62,012 protein-coding genes were predicted, assisted by transcriptome sequencing. In addition, we are reporting an efficient method for extracting high-molecular-weight DNA from dormant buds, by which contamination by environmental bacteria and fungi was kept at a minimum. CONCLUSIONS: The assembled genome will be a valuable resource and reference for future population genomics studies on the evolution and past climate change adaptation of beech and will be helpful for identifying genes, e.g., involved in drought tolerance, in order to select and breed individuals to adapt forestry to climate change in Europe. A continuously updated genome browser and download page can be accessed from beechgenome.net, which will include future genome versions of the reference individual Bhaga, as new sequencing approaches develop.
1000 Sacherschließung
lokal genomics
lokal forest tree
lokal hybrid assembly
lokal fungi
lokal transcriptomics
lokal hardwood
1000 Fächerklassifikation (DDC)
1000 Liste der Beteiligten
  1. https://frl.publisso.de/adhoc/uri/TWlzaHJhLCBCYWdkZXZp|https://frl.publisso.de/adhoc/uri/R3VwdGEsIERlZXBhayBLLg==|https://orcid.org/0000-0002-1547-7245|https://orcid.org/0000-0002-4668-7552|https://orcid.org/0000-0003-3480-2936|https://frl.publisso.de/adhoc/uri/TmFtLCBCb3Jh|https://orcid.org/0000-0001-5375-7689|https://orcid.org/0000-0002-9660-6343|https://orcid.org/0000-0003-2981-1205|https://orcid.org/0000-0003-3923-5424|https://orcid.org/0000-0002-6899-2523|https://orcid.org/0000-0001-7740-6875
1000 Label
1000 Förderer
  1. Hessisches Ministerium für Wissenschaft und Kunst |
  2. Narodowe Centrum Nauki |
1000 Fördernummer
  1. -
  2. 2012/04/A/NZ9/00500
1000 Förderprogramm
  1. Landes-Offensive zur Entwicklung Wissenschaftlich-ökonomischer Exzellenz (LOEWE)
  2. -
1000 Dateien
  1. A reference genome of the European beech (Fagus sylvatica L.)
1000 Förderung
  1. 1000 joinedFunding-child
    1000 Förderer Hessisches Ministerium für Wissenschaft und Kunst |
    1000 Förderprogramm Landes-Offensive zur Entwicklung Wissenschaftlich-ökonomischer Exzellenz (LOEWE)
    1000 Fördernummer -
  2. 1000 joinedFunding-child
    1000 Förderer Narodowe Centrum Nauki |
    1000 Förderprogramm -
    1000 Fördernummer 2012/04/A/NZ9/00500
1000 Objektart article
1000 Beschrieben durch
1000 @id frl:6425156.rdf
1000 Erstellt am 2021-01-12T09:50:15.238+0100
1000 Erstellt von 270
1000 beschreibt frl:6425156
1000 Bearbeitet von 122
1000 Zuletzt bearbeitet 2021-02-19T08:35:39.492+0100
1000 Objekt bearb. Fri Feb 19 08:00:20 CET 2021
1000 Vgl. frl:6425156
1000 Oai Id
  1. oai:frl.publisso.de:frl:6425156 |
1000 Sichtbarkeit Metadaten public
1000 Sichtbarkeit Daten public
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