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1000
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Abstract/Summary
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Injury of vascular endothelial cell is one of the main factors triggering atherosclerosis. Peptide AAGALPS was derived from digestion and absorption product of rice α-globulin, which was proved to prevent atherosclerosis in previous study. This study aims to investigate the potential effects of AAGALPS on improving tumor necrosis factor-α (TNF-α)-stimulated human umbilical vein endothelial cells’ (HUVECs) injury. As a result, the viability of HUVECs stimulated by tumor necrosis factor-α was significantly increased by AAGALPS in a dose-dependent manner until 25 μg/ml. The peptide obviously reduced the levels of intercellular adhesion molecule-1, vascular cell adhesion molecule-1, nitric oxide, inducible nitric oxide synthase, reactive oxygen species, and malondialdehyde and increased the concentrations of glutathione peroxidase. Furthermore, AAGALPS inhibited the nuclear factor κB (NF-κB) activation and nuclear translocation through regulating inhibitor of nuclear factor κB kinase α and inhibitor of NF-κB. These results indicated that AAGALPS protected vascular endothelial cells through mediating inflammation and oxidative stress.
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