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Environmental DNA - 2021 - Wilting - Creating genetic reference datasets Indirect sampling of target species using.pdf 2,16MB
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1000 Titel
  • Creating genetic reference datasets: Indirect sampling of target species using terrestrial leeches as sample “collectors”
1000 Autor/in
  1. Wilting, Andreas |
  2. Van Nguyen, Thanh |
  3. Axtner, Jan |
  4. Nguyen, An |
  5. Schmidt, Anke |
  6. Le, Minh |
  7. Nguyen, Anh H. Q. |
  8. Rawson, Benjamin |
  9. Tilker, Andrew |
  10. Fickel, Joerns |
1000 Erscheinungsjahr 2021
1000 LeibnizOpen
1000 Publikationstyp
  1. Artikel |
1000 Online veröffentlicht
  • 2021-09-27
1000 Erschienen in
1000 Quellenangabe
  • 4(2):311-325
1000 FRL-Sammlung
1000 Copyrightjahr
  • 2021
1000 Lizenz
1000 Verlagsversion
  • https://doi.org/10.1002/edn3.256 |
1000 Ergänzendes Material
  • https://onlinelibrary.wiley.com/doi/full/10.1002/edn3.256#support-information-section |
1000 Publikationsstatus
1000 Begutachtungsstatus
1000 Sprache der Publikation
1000 Abstract/Summary
  • Sampling genetic material from rare and often secretive mammals can be difficult in challenging environments such as tropical rainforests. Large-scale sampling is important however for resolving species' taxonomic uncertainties, as well as to help provide genetic material for reference databases that can be used with DNA forensics to combat the illegal wildlife trade. Environmental DNA (eDNA) and invertebrate-derived DNA (iDNA) offer a promising way to overcome this sampling bottleneck, though to date e/iDNA studies have been primarily used to obtain information about species presence using barcoding or metabarcoding approaches. In this study, we collected 4,710 terrestrial leeches across six protected areas in the Annamite Mountains of Viet Nam and Laos and sequenced fragments (223–694 bp) of mt-Cytb and/or mt-Dcr of the mitogenomes from six species that are frequently found in the illegal wildlife trade. Although amplification success was generally low (<50%) because DNA was highly degraded, we showed that e/iDNA samples can be an important source of genetic material for difficult to sample species and show promise as a way to fill existing sampling gaps. Because e/iDNA sampling has the potential to generate much-needed genetic data to improve taxonomic assessments, this approach can be used to support conservation management by contributing to genetic databases that are necessary to determine the origin of wildlife products or to inform the reintroduction of confiscated animals.
1000 Sacherschließung
lokal invertebrate-derived DNA (iDNA)
lokal Southeast Asia
lokal phylogeography
lokal wildlife trade
lokal DNA forensics
1000 Fächerklassifikation (DDC)
1000 Liste der Beteiligten
  1. https://orcid.org/0000-0001-5073-9186|https://orcid.org/0000-0002-8057-7912|https://orcid.org/0000-0003-1269-5586|https://orcid.org/0000-0003-0456-3866|https://frl.publisso.de/adhoc/uri/U2NobWlkdCwgQW5rZQ==|https://orcid.org/0000-0002-2953-2815|https://frl.publisso.de/adhoc/uri/Tmd1eWVuLCBBbmggSC4gUS4=|https://orcid.org/0000-0003-4141-5985|https://orcid.org/0000-0003-3630-8691|https://orcid.org/0000-0002-0593-5820
1000 Label
1000 Förderer
  1. Point Defiance Zoo and Aquarium |
  2. Safari Club International Foundation |
  3. Leibniz-Institut für Zoo- und Wildtierforschung |
  4. GreaterGood |
  5. Ocean Park Conservation Foundation, Hong Kong |
  6. Russell E. Train Education for Nature |
  7. National Geographic Society |
  8. Critical Ecosystem Partnership Fund |
  9. Bundesministerium für Bildung und Forschung |
  10. Mohamed bin Zayed Species Conservation Fund |
1000 Fördernummer
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  9. 01LN1301A
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1000 Förderprogramm
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1000 Dateien
  1. Creating genetic reference datasets: Indirect sampling of target species using terrestrial leeches as sample “collectors”
1000 Förderung
  1. 1000 joinedFunding-child
    1000 Förderer Point Defiance Zoo and Aquarium |
    1000 Förderprogramm -
    1000 Fördernummer -
  2. 1000 joinedFunding-child
    1000 Förderer Safari Club International Foundation |
    1000 Förderprogramm -
    1000 Fördernummer -
  3. 1000 joinedFunding-child
    1000 Förderer Leibniz-Institut für Zoo- und Wildtierforschung |
    1000 Förderprogramm -
    1000 Fördernummer -
  4. 1000 joinedFunding-child
    1000 Förderer GreaterGood |
    1000 Förderprogramm -
    1000 Fördernummer -
  5. 1000 joinedFunding-child
    1000 Förderer Ocean Park Conservation Foundation, Hong Kong |
    1000 Förderprogramm -
    1000 Fördernummer -
  6. 1000 joinedFunding-child
    1000 Förderer Russell E. Train Education for Nature |
    1000 Förderprogramm -
    1000 Fördernummer -
  7. 1000 joinedFunding-child
    1000 Förderer National Geographic Society |
    1000 Förderprogramm -
    1000 Fördernummer -
  8. 1000 joinedFunding-child
    1000 Förderer Critical Ecosystem Partnership Fund |
    1000 Förderprogramm -
    1000 Fördernummer -
  9. 1000 joinedFunding-child
    1000 Förderer Bundesministerium für Bildung und Forschung |
    1000 Förderprogramm -
    1000 Fördernummer 01LN1301A
  10. 1000 joinedFunding-child
    1000 Förderer Mohamed bin Zayed Species Conservation Fund |
    1000 Förderprogramm -
    1000 Fördernummer -
1000 Objektart article
1000 Beschrieben durch
1000 @id frl:6434466.rdf
1000 Erstellt am 2022-08-04T12:12:22.605+0200
1000 Erstellt von 317
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1000 Bearbeitet von 317
1000 Zuletzt bearbeitet 2022-08-04T12:13:38.287+0200
1000 Objekt bearb. Thu Aug 04 12:13:10 CEST 2022
1000 Vgl. frl:6434466
1000 Oai Id
  1. oai:frl.publisso.de:frl:6434466 |
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1000 Sichtbarkeit Daten public
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