4-hydroxytamoxifen does not deteriorate cardiac function in cardiomyocyte-specific MerCreMer transgenic mice

  1. Heinen, Andre
  2. Gödecke, Stefanie
  3. Flögel, Ulrich
  4. Miklos, Dominika
  5. Bottermann, Katharina
  6. Spychala, André
  7. Gödecke, Axel ORCID logo

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WeightNameValue
1000 Titel
  • 4-hydroxytamoxifen does not deteriorate cardiac function in cardiomyocyte-specific MerCreMer transgenic mice
1000 Autor/in
  1. Heinen, Andre |
  2. Gödecke, Stefanie |
  3. Flögel, Ulrich |
  4. Miklos, Dominika |
  5. Bottermann, Katharina |
  6. Spychala, André |
  7. Gödecke, Axel |
1000 Erscheinungsjahr 2021
1000 Publikationstyp
  1. Artikel |
1000 Online veröffentlicht
  • 2021-02-05
1000 Erschienen in
1000 Quellenangabe
  • 116(1):8
1000 Copyrightjahr
  • 2021
1000 Lizenz
1000 Verlagsversion
  • https://doi.org/10.1007/s00395-020-00841-9 |
  • https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7864833/ |
1000 Publikationsstatus
1000 Begutachtungsstatus
1000 Sprache der Publikation
1000 Abstract/Summary
  • Conditional, cell-type-specific transgenic mouse lines are of high value in cardiovascular research. A standard tool for cardiomyocyte-restricted DNA editing is the αMHC-MerCreMer/loxP system. However, there is an ongoing debate on the occurrence of cardiac side effects caused by unspecific Cre activity or related to tamoxifen/oil overload. Here, we investigated potential adverse effects of DNA editing by the αMHC-MerCreMer/loxP system in combination with a low-dose treatment protocol with the tamoxifen metabolite 4-hydroxytamoxifen (OH-Txf). αMHC-MerCreMer mice received intraperitoneally OH-Txf (20 mg/kg) for 5 or 10 days. These treatment protocols were highly efficient to induce DNA editing in adult mouse hearts. Multi-parametric magnetic resonance imaging revealed neither transient nor permanent effects on cardiac function during or up to 19 days after 5 day OH-Txf treatment. Furthermore, OH-Txf did not affect cardiac phosphocreatine/ATP ratios assessed by in vivo
1000 Sacherschließung
lokal Cardiac energetics
lokal Cardiac function
lokal Mice, Inbred C57BL [MeSH]
lokal Glycogen Synthase Kinase 3 beta/genetics [MeSH]
lokal Original Contribution
lokal Tamoxifen/pharmacology [MeSH]
lokal Mice, Transgenic [MeSH]
lokal Proto-Oncogene Proteins c-akt/genetics [MeSH]
lokal Gene Expression Regulation/drug effects [MeSH]
lokal Male [MeSH]
lokal Myocytes, Cardiac/metabolism [MeSH]
lokal Tamoxifen/toxicity [MeSH]
lokal Energy Metabolism/drug effects [MeSH]
lokal Cardiomyopathy
lokal Integrases/genetics [MeSH]
lokal Myocytes, Cardiac/drug effects [MeSH]
lokal Myosin Heavy Chains/genetics [MeSH]
lokal p38 Mitogen-Activated Protein Kinases/genetics [MeSH]
lokal Tamoxifen/analogs
lokal aMHC-MerCreMer/loxP system
lokal Gene Editing [MeSH]
lokal Fibrosis [MeSH]
lokal Ventricular Remodeling/drug effects [MeSH]
lokal Animals [MeSH]
lokal Myocytes, Cardiac/pathology [MeSH]
lokal Time Factors [MeSH]
lokal Proto-Oncogene Proteins c-akt/metabolism [MeSH]
lokal p38 Mitogen-Activated Protein Kinases/metabolism [MeSH]
lokal Ventricular Function, Left/drug effects [MeSH]
lokal 4-hydroxytamoxifen
lokal Glycogen Synthase Kinase 3 beta/metabolism [MeSH]
1000 Liste der Beteiligten
  1. https://frl.publisso.de/adhoc/uri/SGVpbmVuLCBBbmRyZQ==|https://frl.publisso.de/adhoc/uri/R8O2ZGVja2UsIFN0ZWZhbmll|https://frl.publisso.de/adhoc/uri/RmzDtmdlbCwgVWxyaWNo|https://frl.publisso.de/adhoc/uri/TWlrbG9zLCBEb21pbmlrYQ==|https://frl.publisso.de/adhoc/uri/Qm90dGVybWFubiwgS2F0aGFyaW5h|https://frl.publisso.de/adhoc/uri/U3B5Y2hhbGEsIEFuZHLDqQ==|https://orcid.org/0000-0001-9927-130X
1000 Hinweis
  • DeepGreen-ID: b98e742b75a04edfb428514778371afd ; metadata provieded by: DeepGreen (https://www.oa-deepgreen.de/api/v1/), LIVIVO search scope life sciences (http://z3950.zbmed.de:6210/livivo), Crossref Unified Resource API (https://api.crossref.org/swagger-ui/index.html), to.science.api (https://frl.publisso.de/), ZDB JSON-API (beta) (https://zeitschriftendatenbank.de/api/), lobid - Dateninfrastruktur für Bibliotheken (https://lobid.org/resources/search)
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1000 Erstellt am 2023-05-11T10:58:59.173+0200
1000 Erstellt von 322
1000 beschreibt frl:6450770
1000 Zuletzt bearbeitet 2023-10-21T04:01:46.554+0200
1000 Objekt bearb. Sat Oct 21 04:01:46 CEST 2023
1000 Vgl. frl:6450770
1000 Oai Id
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